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Characterization of the genomic and transcriptional structure of the CRX gene : substantial differences between human and mouse.

Hodges, Matt and Vieira, Helena and Gregory-Evans, Kevin and Gregory-Evans, Cheryl Y. (2002) Characterization of the genomic and transcriptional structure of the CRX gene : substantial differences between human and mouse. Genomics, 80 (5). pp. 531-542. ISSN 0888-7543

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Abstract

We have previously shown that there is a temporal difference in human CRX: gene expression compared with that of mouse Crx. We have now characterized these genes at the genomic and transcriptional levels and here we expand on this earlier report. Human CRX: spans 25 kb and has six exons, and mouse Crx: spans 15 kb and has four exons. We isolated seven human and two mouse mRNAs generated by alternative splicing of a variable 5′ untranslated region. The human and mouse genes share an evolutionarily conserved promoter, which contains OTX/CRX type and SP1/AP2 binding sites and drives expression of two conserved transcripts in both species. Additionally, the human gene has a second human-specific promoter, which has OTX/CRX type binding sites and drives expression of five other transcripts. Band shift assays have shown that six of the seven candidate OTX/CRX elements bind CRX in vitro, possibly implying that the gene can regulate its own expression. These data may account for the differences in temporal expression in vivo: we have previously reported between these two species.

Item Type: Journal Article
Journal or Publication Title: Genomics
Uncontrolled Keywords: CRX ; eye
Subjects:
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 65575
Deposited By: ep_importer_pure
Deposited On: 09 Jul 2013 09:06
Refereed?: Yes
Published?: Published
Last Modified: 18 Sep 2018 04:08
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/65575

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