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Mechanistic insights into nanotoxicity determined by synchrotron radiation-based Fourier-transform infrared imaging and multivariate analysis

Riding, Matthew J. and Trevisan, Júlio and Hirschmugl, Carol J. and Jones, Kevin C. and Semple, Kirk T. and Martin, Francis L. (2012) Mechanistic insights into nanotoxicity determined by synchrotron radiation-based Fourier-transform infrared imaging and multivariate analysis. Environment International, 50. pp. 56-65. ISSN 0160-4120

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Abstract

Our ability to identify the mechanisms by which carbon-based nanomaterials (CBNs) exert toxicity in cells is constrained by the lack of standardized methodologies to assay endpoint effects. Herein we describe a method of mechanistically identifying the effects of various CBN types in both prokaryotic and eukaryotic cells using multi-beam synchrotron radiation-based Fourier-transform infrared imaging (SR-FTIRI) at diffraction-limited resolution. This technique overcomes many of the inherent difficulties of assaying nanotoxicity and demonstrates exceptional sensitivity in identifying the effects of CBNs in cells at environmentally-relevant concentrations. We identify key mechanisms of nanotoxicity as the alteration of Amide and lipid biomolecules, but propose more specific bioactivity of CBNs occurs as a result of specific interactions between CBN structural conformation and cellular characteristics.

Item Type: Article
Journal or Publication Title: Environment International
Uncontrolled Keywords: Carbon nanoparticles ; Multivariate analysis ; Nanotoxicity ; Spectroscopy ; Synchrotron radiation
Subjects: UNSPECIFIED
Departments: Faculty of Science and Technology > Lancaster Environment Centre
Faculty of Science and Technology > School of Computing & Communications
ID Code: 60253
Deposited By: ep_importer_pure
Deposited On: 26 Nov 2012 11:44
Refereed?: Yes
Published?: Published
Last Modified: 01 Oct 2013 15:30
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/60253

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