Riding, Matthew J. and Trevisan, Júlio and Hirschmugl, Carol J. and Jones, Kevin C. and Semple, Kirk T. and Martin, Francis L. (2012) Mechanistic insights into nanotoxicity determined by synchrotron radiation-based Fourier-transform infrared imaging and multivariate analysis. Environment International, 50. pp. 56-65. ISSN 0160-4120Full text not available from this repository.
Our ability to identify the mechanisms by which carbon-based nanomaterials (CBNs) exert toxicity in cells is constrained by the lack of standardized methodologies to assay endpoint effects. Herein we describe a method of mechanistically identifying the effects of various CBN types in both prokaryotic and eukaryotic cells using multi-beam synchrotron radiation-based Fourier-transform infrared imaging (SR-FTIRI) at diffraction-limited resolution. This technique overcomes many of the inherent difficulties of assaying nanotoxicity and demonstrates exceptional sensitivity in identifying the effects of CBNs in cells at environmentally-relevant concentrations. We identify key mechanisms of nanotoxicity as the alteration of Amide and lipid biomolecules, but propose more specific bioactivity of CBNs occurs as a result of specific interactions between CBN structural conformation and cellular characteristics.
|Journal or Publication Title:||Environment International|
|Uncontrolled Keywords:||Carbon nanoparticles ; Multivariate analysis ; Nanotoxicity ; Spectroscopy ; Synchrotron radiation|
|Departments:||Faculty of Science and Technology > Lancaster Environment Centre|
Faculty of Science and Technology > School of Computing & Communications
|Deposited On:||26 Nov 2012 11:44|
|Last Modified:||07 Jan 2015 18:05|
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