Riding, Matthew J. and Trevisan, Júlio and Hirschmugl, Carol J. and Jones, Kevin C. and Semple, Kirk T. and Martin, Francis L. (2012) Mechanistic insights into nanotoxicity determined by synchrotron radiation-based Fourier-transform infrared imaging and multivariate analysis. Environment International, 50. pp. 56-65. ISSN 0160-4120
Full text not available from this repository.Abstract
Our ability to identify the mechanisms by which carbon-based nanomaterials (CBNs) exert toxicity in cells is constrained by the lack of standardized methodologies to assay endpoint effects. Herein we describe a method of mechanistically identifying the effects of various CBN types in both prokaryotic and eukaryotic cells using multi-beam synchrotron radiation-based Fourier-transform infrared imaging (SR-FTIRI) at diffraction-limited resolution. This technique overcomes many of the inherent difficulties of assaying nanotoxicity and demonstrates exceptional sensitivity in identifying the effects of CBNs in cells at environmentally-relevant concentrations. We identify key mechanisms of nanotoxicity as the alteration of Amide and lipid biomolecules, but propose more specific bioactivity of CBNs occurs as a result of specific interactions between CBN structural conformation and cellular characteristics.
| Item Type: | Article |
|---|---|
| Journal or Publication Title: | Environment International |
| Uncontrolled Keywords: | Carbon nanoparticles ; Multivariate analysis ; Nanotoxicity ; Spectroscopy ; Synchrotron radiation |
| Subjects: | UNSPECIFIED |
| Departments: | Faculty of Science and Technology > Lancaster Environment Centre Faculty of Science and Technology > School of Computing & Communications |
| ID Code: | 60253 |
| Deposited By: | ep_importer_pure |
| Deposited On: | 26 Nov 2012 11:44 |
| Refereed?: | Yes |
| Published?: | Published |
| Last Modified: | 15 May 2013 04:45 |
| Identification Number: | |
| URI: | http://eprints.lancs.ac.uk/id/eprint/60253 |
Actions (login required)
| View Item |
