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Concentration-dependent effects of carbon nanoparticles in gram-negative bacteria determined by infrared spectroscopy with multivariate analysis

Riding, Matthew J. and Martin, Francis L. and Trevisan, Julio and Llabjani, Valon and Patel, Imran I. and Jones, Kevin C. and Semple, Kirk T. (2012) Concentration-dependent effects of carbon nanoparticles in gram-negative bacteria determined by infrared spectroscopy with multivariate analysis. Environmental Pollution, 163. pp. 226-234. ISSN 0269-7491

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Abstract

With increasing production of carbon nanoparticles (CNPs), environmental release of these entities becomes an ever-greater inevitability. However, many questions remain regarding their impact on soil microorganisms. This study examined the effects of long or short multiwalled carbon nanotubes (MWCNTs), C60 fullerene and fullerene soot in Gram-negative bacteria. Attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy was applied to derive signature spectral fingerprints of effects. A concentration-dependent response in spectral alterations was observed for each nanoparticle type. Long or short MWCNTs and fullerene soot gave rise to similar alterations to lipids, Amide II and DNA. The extent of alteration varies with nanoparticle size, with smaller short MWCNTs resulting in greater toxicity than long MWCNTs. Fullerene soot was the least toxic. C60 results in the most distinct and largest overall alterations, notably in extensive protein alteration. This work demonstrates a novel approach for assaying and discriminating the effects of CNPs in target systems. (C) 2011 Elsevier Ltd. All rights reserved.

Item Type: Article
Journal or Publication Title: Environmental Pollution
Uncontrolled Keywords: Carbon nanoparticles ; Gram-negative bacteria ; Infrared spectroscopy ; Multivariate analysis ; Nanotoxicity
Subjects:
Departments: Faculty of Science and Technology > Lancaster Environment Centre
Faculty of Science and Technology > School of Computing & Communications
ID Code: 58439
Deposited By: ep_importer_pure
Deposited On: 18 Sep 2012 16:01
Refereed?: Yes
Published?: Published
Last Modified: 01 Oct 2013 15:28
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/58439

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