Trevisan, Julio and Angelov, Plamen P. and Patel, Imran I. and Najand, Ghazal M. and Cheung, Karen T. and Llabjani, Valon and Pollock, Hubert M. and Bruce, Shannon W. and Pant, Kamala and Carmichael, Paul L. and Martin, Francis L. (2010) Syrian hamster embryo (SHE) assay (pH 6.7) coupled with infrared spectroscopy and chemometrics towards toxicological assessment. Analyst, 135 (12). pp. 3266-3272. ISSN 0003-2654Full text not available from this repository.
The Syrian hamster embryo (SHE) assay (pH 6.7) is an in vitro candidate to replace in vivo carcinogenicity tests. However, the conventional method of visual scoring of foci (non-transformed vs. transformed colonies) can be time-consuming and is open to subjectivity. Infrared (IR) spectroscopy has the potential to provide objective assessment of such SHE colonies with the added advantage of potentially providing mechanistic information. In this study, SHE cells were treated with one of eight different chemical regimens, allowed in culture to attach and form foci on IR-reflective glass slides; these were subsequently interrogated by attenuated total reflection (ATR) Fourier-transform IR (FTIR) spectroscopy. Derived mid-IR spectra (n = 13 406) were subjected to chemometric analysis focusing primarily on the extraction of biochemical information related to test agent treatment and/or morphological transformation. The use of ATR-FTIR spectroscopy with chemometrics to analyze the SHE assay is a novel approach to toxicological assessment.
|Journal or Publication Title:||Analyst|
|Departments:||Faculty of Science and Technology > School of Computing & Communications|
Faculty of Science and Technology > Lancaster Environment Centre
Faculty of Health and Medicine > Biomedical & Life Sciences
Faculty of Science and Technology > Physics
|Deposited On:||19 Sep 2012 12:28|
|Last Modified:||23 Oct 2012 10:26|
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