Dillon, R. J. and Lane, R. P. (1993) Bloodmeal digestion in the midgut of Phlebotomus papatasi and Phlebotomus langeroni. Medical and Veterinary Entomology, 7 (3). pp. 225-232. ISSN 0269-283XFull text not available from this repository.
Bloodmeal digestion in midguts of the sandflies Phlebotomus papatasi and Phlebotomus langeroni (Diptera: Psychodidae) was investigated in optimized assays to detect general protease, trypsin and aminopeptidase activities using synthetic substrates. Optimal activity occurred at pH 8-9 for all enzymes examined in both species. Protease activity peaked at 24-34 h post human bloodmeal in midguts of P.papatasi and 34-48 h in P. langeroni; all endo- and exoprotease activities were completed by 50 h in P.papatasi compared to 72 h in P.langeroni. Hydrolysis of two chymotrypsin substrates was <2% of trypsin activity in both species. Aminopeptidase activity was associated mainly with the midgut wall, whereas trypsin activity was confined to the midgut lumen. A feature of digestion in P.langeroni was the high level of aminopeptidase recorded within 10 h of the bloodmeal.
|Journal or Publication Title:||Medical and Veterinary Entomology|
|Uncontrolled Keywords:||Phlebotomus papatasi ; Phlebotomus langeroni ; sandfly ; blood digestion ; midgut ; protease|
|Subjects:||Q Science > QR Microbiology|
|Departments:||Faculty of Health and Medicine > Biomedical & Life Sciences|
|Deposited On:||19 Oct 2012 11:00|
|Last Modified:||07 Jan 2015 17:51|
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