Dillon, R. J. and Lane, R. P. (1993) Bloodmeal digestion in the midgut of Phlebotomus papatasi and Phlebotomus langeroni. Medical and veterinary entomology, 7 (3). pp. 225-232. ISSN 0269-283X
Full text not available from this repository.Abstract
Bloodmeal digestion in midguts of the sandflies Phlebotomus papatasi and Phlebotomus langeroni (Diptera: Psychodidae) was investigated in optimized assays to detect general protease, trypsin and aminopeptidase activities using synthetic substrates. Optimal activity occurred at pH 8-9 for all enzymes examined in both species. Protease activity peaked at 24-34 h post human bloodmeal in midguts of P.papatasi and 34-48 h in P. langeroni; all endo- and exoprotease activities were completed by 50 h in P.papatasi compared to 72 h in P.langeroni. Hydrolysis of two chymotrypsin substrates was <2% of trypsin activity in both species. Aminopeptidase activity was associated mainly with the midgut wall, whereas trypsin activity was confined to the midgut lumen. A feature of digestion in P.langeroni was the high level of aminopeptidase recorded within 10 h of the bloodmeal.
| Item Type: | Article |
|---|---|
| Journal or Publication Title: | Medical and veterinary entomology |
| Uncontrolled Keywords: | Phlebotomus papatasi ; Phlebotomus langeroni ; sandfly ; blood digestion ; midgut ; protease |
| Subjects: | Q Science > QR Microbiology |
| Departments: | Faculty of Health and Medicine > Biomedical & Life Sciences |
| ID Code: | 57959 |
| Deposited By: | ep_importer_pure |
| Deposited On: | 19 Oct 2012 11:00 |
| Refereed?: | Yes |
| Published?: | Published |
| Last Modified: | 19 Oct 2012 11:00 |
| Identification Number: | |
| URI: | http://eprints.lancs.ac.uk/id/eprint/57959 |
Actions (login required)
| View Item |
