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Bloodmeal digestion in the midgut of Phlebotomus papatasi and Phlebotomus langeroni

Dillon, R. J. and Lane, R. P. (1993) Bloodmeal digestion in the midgut of Phlebotomus papatasi and Phlebotomus langeroni. Medical and veterinary entomology, 7 (3). pp. 225-232. ISSN 0269-283X

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Abstract

Bloodmeal digestion in midguts of the sandflies Phlebotomus papatasi and Phlebotomus langeroni (Diptera: Psychodidae) was investigated in optimized assays to detect general protease, trypsin and aminopeptidase activities using synthetic substrates. Optimal activity occurred at pH 8-9 for all enzymes examined in both species. Protease activity peaked at 24-34 h post human bloodmeal in midguts of P.papatasi and 34-48 h in P. langeroni; all endo- and exoprotease activities were completed by 50 h in P.papatasi compared to 72 h in P.langeroni. Hydrolysis of two chymotrypsin substrates was <2% of trypsin activity in both species. Aminopeptidase activity was associated mainly with the midgut wall, whereas trypsin activity was confined to the midgut lumen. A feature of digestion in P.langeroni was the high level of aminopeptidase recorded within 10 h of the bloodmeal.

Item Type: Article
Journal or Publication Title: Medical and veterinary entomology
Uncontrolled Keywords: Phlebotomus papatasi ; Phlebotomus langeroni ; sandfly ; blood digestion ; midgut ; protease
Subjects: Q Science > QR Microbiology
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 57959
Deposited By: ep_importer_pure
Deposited On: 19 Oct 2012 11:00
Refereed?: Yes
Published?: Published
Last Modified: 19 Oct 2012 11:00
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/57959

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