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Opsonization of polyethylene wear particles regulates macrophage and osteoblast responses in vitro

Elfick, A. P. D. and Green, S. M. and McCaskie, A. W. and Birch, M. A. (2004) Opsonization of polyethylene wear particles regulates macrophage and osteoblast responses in vitro. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 71B (2). pp. 244-251. ISSN 1552-4973

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Abstract

The cellular reaction to wear debris may result in the failure of an artificial joint's fixation to the skeleton. The influence of debris opsinization on cell activity has received little attention. This study seeks to establish whether different proteinaceous culture environments may invoke variant cellular responses to debris challenge. Consideration of the zeta potential of a low density polyethylene particle group and an ex vitro ultrahigh molecular weight polyethylene particle group revealed that the nature of the protein adsorbants is related to the concentration of the proteins in solution. Furthermore, the composition of the adsorbed layer was shown to vary with the spectra of proteins in solution. In standard cell culture conditions zeta potential approached zero, indicating the high probability of particle agglomeration. Cell challenge studies with U937 macrophages showed that BSA and FCS protein adsorption mediated increased cell adhesion, while bovine IgG showed little change over control values. No changes in behavior of osteoblastic cells were observed in similar experiments.

Item Type: Article
Journal or Publication Title: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
Subjects: T Technology > TA Engineering (General). Civil engineering (General)
Departments: Faculty of Science and Technology > Engineering
ID Code: 57912
Deposited By: ep_importer_pure
Deposited On: 17 Oct 2012 10:02
Refereed?: Yes
Published?: Published
Last Modified: 10 Apr 2014 00:05
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/57912

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