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Reactive Oxygen Species-mediated Immunity against Leishmania mexicana and Serratia marcescens in the Phlebotomine Sand Fly Lutzomyia longipalpis

Diaz-Albiter, Hector and Sant'anna, Mauricio R V and Genta, Fernando A and Dillon, Rod J (2012) Reactive Oxygen Species-mediated Immunity against Leishmania mexicana and Serratia marcescens in the Phlebotomine Sand Fly Lutzomyia longipalpis. Journal of Biological Chemistry, 287 (28). pp. 23995-24003. ISSN 0021-9258

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Abstract

Phlebotomine sand flies are the vectors of medically important Leishmania. The Leishmania protozoa reside in the sand fly gut, but the nature of the immune response to the presence of Leishmania is unknown. Reactive oxygen species (ROS) are a major component of insect innate immune pathways regulating gut-microbe homeostasis. Here we show that the concentration of ROS increased in sand fly midguts after they fed on the insect pathogen Serratia marcescens but not after feeding on the Leishmania that uses the sand fly as a vector. Moreover, the Leishmania is sensitive to ROS either by oral administration of ROS to the infected fly or by silencing a gene that expresses a sand fly ROS-scavenging enzyme. Finally, the treatment of sand flies with an exogenous ROS scavenger (uric acid) altered the gut microbial homeostasis, led to an increased commensal gut microbiota, and reduced insect survival after oral infection with S. marcescens. Our study demonstrates a differential response of the sand fly ROS system to gut microbiota, an insect pathogen, and the Leishmania that utilize the sand fly as a vehicle for transmission between mammalian hosts.

Item Type: Article
Journal or Publication Title: Journal of Biological Chemistry
Uncontrolled Keywords: Innate Immunity ; Insect immunity ; Leishmania ; Parasitology ; Reactive Oxygen Species (ROS) ; Lutzomyia ; Sand Fly
Subjects: Q Science > QR Microbiology > QR355 Virology
Departments: UNSPECIFIED
ID Code: 56673
Deposited By: ep_importer_pure
Deposited On: 10 Aug 2012 18:32
Refereed?: Yes
Published?: Published
Last Modified: 23 Jul 2014 15:03
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/56673

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