Lancaster EPrints

Polymorphic microsatellite repeats are not conserved between Leishmania donovani and Leishmania major

Jamjoom, Manal and Ashford, R W and Bates, Paul and Kemp, Stephen J and Noyes, Harry A (2002) Polymorphic microsatellite repeats are not conserved between Leishmania donovani and Leishmania major. Molecular Ecology Notes, 2 (2). pp. 104-106.

Full text not available from this repository.

Abstract

Thirteen sets of polymerase chain reaction (PCR) primers were designed to amplify microsatellite loci identified in the genome sequence of Leishmania major. Polymorphisms were detected in L. major at all loci. In Leishmania donovani only two of these loci were informative for classification purposes with this data set. The PCR products of all loci from one L. donovani strain were sequenced and it was found that the number of repeats in the microsatellite loci were either substantially reduced with respect to L. major or absent altogether. Consequently it is unlikely to be possible to use the genome sequence of L. major to identify polymorphic microsatellite loci in other Leishmania species.

Item Type: Article
Journal or Publication Title: Molecular Ecology Notes
Uncontrolled Keywords: Leishmania tropica ; Leishmania mexicana ; mapping ; Leishmania infantum
Subjects: Q Science > QR Microbiology > QR355 Virology
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 56306
Deposited By: ep_importer_pure
Deposited On: 27 Jul 2012 16:33
Refereed?: Yes
Published?: Published
Last Modified: 27 Jul 2012 16:33
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/56306

Actions (login required)

View Item