Dluzewski, A R and Ling, I T and Rangachari, K and Bates, P A and Wilson, R J (1984) A simple method for isolating viable mature parasites of Plasmodium falciparum from cultures. Transactions of the Royal Society of Tropical Medicine and Hygiene, 78 (5). pp. 622-624. ISSN 0035-9203Full text not available from this repository.
Mature asexual parasites from cultures of knobby or knobless clones of P. falciparum containing 5 to 10% late forms were harvested by layering up to 10(9) erythrocytes on 3 ml Percoll. The density of the Percoll was adjusted to between 1.081 and 1.091 g cm-3, depending on the maturity of the parasites. Centrifugation at 1500 g for 10 min produced a sharp band at the interface containing mature parasites with a purity averaging 86%. Washed parasites derived from the layer or pellet showed good viability in vitro.
|Journal or Publication Title:||Transactions of the Royal Society of Tropical Medicine and Hygiene|
|Subjects:||Q Science > QR Microbiology > QR355 Virology|
|Departments:||Faculty of Health and Medicine > Biomedical & Life Sciences|
|Deposited On:||27 Jul 2012 16:19|
|Last Modified:||09 Apr 2014 23:53|
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