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A simple method for isolating viable mature parasites of Plasmodium falciparum from cultures

Dluzewski, A R and Ling, I T and Rangachari, K and Bates, P A and Wilson, R J (1984) A simple method for isolating viable mature parasites of Plasmodium falciparum from cultures. Transactions of the Royal Society of Tropical Medicine and Hygiene, 78 (5). pp. 622-624. ISSN 0035-9203

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Abstract

Mature asexual parasites from cultures of knobby or knobless clones of P. falciparum containing 5 to 10% late forms were harvested by layering up to 10(9) erythrocytes on 3 ml Percoll. The density of the Percoll was adjusted to between 1.081 and 1.091 g cm-3, depending on the maturity of the parasites. Centrifugation at 1500 g for 10 min produced a sharp band at the interface containing mature parasites with a purity averaging 86%. Washed parasites derived from the layer or pellet showed good viability in vitro.

Item Type: Article
Journal or Publication Title: Transactions of the Royal Society of Tropical Medicine and Hygiene
Subjects: Q Science > QR Microbiology > QR355 Virology
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 56300
Deposited By: ep_importer_pure
Deposited On: 27 Jul 2012 16:19
Refereed?: Yes
Published?: Published
Last Modified: 09 Apr 2014 23:53
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/56300

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