Dluzewski, A R and Ling, I T and Rangachari, K and Bates, P A and Wilson, R J (1984) A simple method for isolating viable mature parasites of Plasmodium falciparum from cultures. Transactions of the Royal Society of Tropical Medicine and Hygiene, 78 (5). pp. 622-624. ISSN 0035-9203
Full text not available from this repository.Abstract
Mature asexual parasites from cultures of knobby or knobless clones of P. falciparum containing 5 to 10% late forms were harvested by layering up to 10(9) erythrocytes on 3 ml Percoll. The density of the Percoll was adjusted to between 1.081 and 1.091 g cm-3, depending on the maturity of the parasites. Centrifugation at 1500 g for 10 min produced a sharp band at the interface containing mature parasites with a purity averaging 86%. Washed parasites derived from the layer or pellet showed good viability in vitro.
| Item Type: | Article |
|---|---|
| Journal or Publication Title: | Transactions of the Royal Society of Tropical Medicine and Hygiene |
| Subjects: | Q Science > QR Microbiology > QR355 Virology |
| Departments: | Faculty of Health and Medicine > Biomedical & Life Sciences |
| ID Code: | 56300 |
| Deposited By: | ep_importer_pure |
| Deposited On: | 27 Jul 2012 16:19 |
| Refereed?: | Yes |
| Published?: | Published |
| Last Modified: | 27 Jul 2012 16:19 |
| Identification Number: | |
| URI: | http://eprints.lancs.ac.uk/id/eprint/56300 |
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