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Nuclear and mitochondrial DNA of the primate malarial parasite Plasmodium knowlesi

Williamson, D H and Wilson, R J and Bates, P A and McCready, S and Perler, F and Qiang, B U (1985) Nuclear and mitochondrial DNA of the primate malarial parasite Plasmodium knowlesi. Molecular and Biochemical Parasitology, 14 (2). pp. 199-209. ISSN 0166-6851

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Abstract

Restriction analyses and DNA/DNA hybridisation of parasite DNA isolated from monkeys infected with the malarial parasite Plasmodium knowlesi has permitted unambiguous identification of the nuclear DNA of this species. Its (G+C) content, as determined by estimations of buoyant density as well as by direct analysis, is about 38%, essentially indistinguishable from that of its primate laboratory host, and grossly different from that of the major human malaria parasite, P. falciparum, which has a (G+C) content of approx. 19%. In addition, gradient fractionation of total P. knowlesi DNA revealed a minor DNA component (approx. 1% of the total) with a (G+C) content of about 19%. This DNA comprises covalently closed circular molecules which have a contour length about 11.6 microns, carry a small cruciform structure, and are thought to originate in the parasite's mitochondria.

Item Type: Article
Journal or Publication Title: Molecular and Biochemical Parasitology
Uncontrolled Keywords: Plasmodium knowlesi ; Malaria ; Nuclear DNA ; Mitochondrial DNAv
Subjects: Q Science > QR Microbiology > QR355 Virology
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 56299
Deposited By: ep_importer_pure
Deposited On: 27 Jul 2012 16:14
Refereed?: Yes
Published?: Published
Last Modified: 27 Jul 2012 16:14
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/56299

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