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Antibodies to the glutamate dehydrogenase of Plasmodium falciparum

Ling, I T and Cooksley, S and Bates, P A and Hempelmann, E and Wilson, R J (1986) Antibodies to the glutamate dehydrogenase of Plasmodium falciparum. Parasitology, 92 (2). pp. 313-324. ISSN 0031-1820

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    Abstract

    Polyclonal antisera raised against Plasmodium knowlesi reacted with NADP-specific glutamate dehydrogenase (GLDH) of P. knowlesi, GLDH of P. falciparum and GLDH of Proteus spp. The antisera did not react with NAD(P) GLDH from bovine liver. Polyclonal antisera raised against the GLDH of Proteus spp. cross-reacted with GLDH from P. falciparum. Monoclonal antibodies (McAbs) obtained from mice immunized with Proteus GLDH were either specific for the bacterial enzyme or cross-reacted with P. falciparum GLDH. The selected McAbs did not react with GLDH from P. knowlesi, P. chabaudi or P. berghei. The GLDH of P. falciparum was shown to be a cytosolic protein (by FAT) with a subunit molecular weight of approximately 49 000 Da (by immunoprecipitation) having a predominantly hexameric form (by sucrose density gradient). Implications of the conserved sequences of GLDHs and other enzymes are discussed.

    Item Type: Article
    Journal or Publication Title: Parasitology
    Additional Information: http://journals.cambridge.org/action/displayJournal?jid=PAR The final, definitive version of this article has been published in the Journal, Parasitology, 92 (2), pp 313-324 1986, © 1986 Cambridge University Press.
    Subjects: Q Science > QR Microbiology > QR355 Virology
    Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
    ID Code: 56298
    Deposited By: ep_importer_pure
    Deposited On: 27 Jul 2012 16:11
    Refereed?: Yes
    Published?: Published
    Last Modified: 09 Apr 2014 23:53
    Identification Number:
    URI: http://eprints.lancs.ac.uk/id/eprint/56298

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