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Mitochondrial DNA of the human malarial parasite Plasmodium falciparum

Gardner, M J and Bates, P A and Ling, I T and Moore, D J and McCready, S and Gunasekera, M B and Wilson, R J and Williamson, D H (1988) Mitochondrial DNA of the human malarial parasite Plasmodium falciparum. Molecular and Biochemical Parasitology, 31 (1). pp. 11-17. ISSN 0166-6851

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Abstract

Covalently closed circular DNA molecules were isolated from Plasmodium falciparum total DNA by isopycnic centrifugation in CsCl gradients containing either ethidium bromide or 2',6-diamidino-2-phenylindole. The circular molecules had an average contour length of 11.1 +/- 0.5 micron, similar to the analogous molecules previously isolated from the simian malaria parasite P. knowlesi. Both circular molecules shared considerable sequence homology and conserved restriction sites. The nucleotide sequence of one 936 bp fragment of the P. falciparum molecule was determined and identified, by a data base homology search, as part of a mitochondrial small rRNA subunit, thus confirming the mitochondrial origin of the circular DNAs of both malarial species.

Item Type: Article
Journal or Publication Title: Molecular and Biochemical Parasitology
Uncontrolled Keywords: Plasmodium falciparum ; Plasmodium knowlesi ; Satellite DNA ; Mitochondrial small subunit rRNA
Subjects: Q Science > QR Microbiology > QR355 Virology
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 56297
Deposited By: ep_importer_pure
Deposited On: 27 Jul 2012 15:15
Refereed?: Yes
Published?: Published
Last Modified: 27 Jul 2012 15:15
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/56297

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