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Expression of D7 and D7-related proteins in the salivary glands of the human malaria mosquito Anopheles stephensi

Suwan, N and Wilkinson, M C and Crampton, J M and Bates, P A (2002) Expression of D7 and D7-related proteins in the salivary glands of the human malaria mosquito Anopheles stephensi. Insect Molecular Biology, 11 (3). pp. 223-232.

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Abstract

Full-length cDNA clones encoding D7 (AnsD7) and D7-related (AnsD7r1) secreted salivary gland proteins were isolated from Anopheles stephensi. Corresponding proteins were separated by SDS-PAGE and analysed by N-terminal sequencing, which also identified a second D7-related protein (AnsD7r2). AnsD7 encodes a protein of 37 kDa, AnsD7r1 of 18 kDa, and AnsD7r2 of 16 kDa. Polyclonal antibodies against recombinant AnsD7 showed immunological cross-reactivity with the D7-related proteins, and alignment demonstrated sequence similarity between the C-terminal region of AnsD7 and the D7-related proteins. AnsD7, AnsD7r1 and AnsD7r2 were major female-specific salivary gland proteins, and Western blotting, immunohistochemistry and immunogold labelling demonstrated expression was predominantly in the secretory cavities of the distal-lateral and median lobes. Expression and localization of D7 and D7-related proteins was similar in Plasmodium berghei-infected and uninfected mosquitoes.

Item Type: Article
Journal or Publication Title: Insect Molecular Biology
Uncontrolled Keywords: Anopheles stephensi ; salivary glands ; mosquito ; Malaria
Subjects: Q Science > QR Microbiology > QR355 Virology
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 56294
Deposited By: ep_importer_pure
Deposited On: 27 Jul 2012 14:59
Refereed?: Yes
Published?: Published
Last Modified: 27 Jul 2012 14:59
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/56294

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