Suwan, N and Wilkinson, M C and Crampton, J M and Bates, P A (2002) Expression of D7 and D7-related proteins in the salivary glands of the human malaria mosquito Anopheles stephensi. Insect Molecular Biology, 11 (3). pp. 223-232. ISSN 1365-2583Full text not available from this repository.
Full-length cDNA clones encoding D7 (AnsD7) and D7-related (AnsD7r1) secreted salivary gland proteins were isolated from Anopheles stephensi. Corresponding proteins were separated by SDS-PAGE and analysed by N-terminal sequencing, which also identified a second D7-related protein (AnsD7r2). AnsD7 encodes a protein of 37 kDa, AnsD7r1 of 18 kDa, and AnsD7r2 of 16 kDa. Polyclonal antibodies against recombinant AnsD7 showed immunological cross-reactivity with the D7-related proteins, and alignment demonstrated sequence similarity between the C-terminal region of AnsD7 and the D7-related proteins. AnsD7, AnsD7r1 and AnsD7r2 were major female-specific salivary gland proteins, and Western blotting, immunohistochemistry and immunogold labelling demonstrated expression was predominantly in the secretory cavities of the distal-lateral and median lobes. Expression and localization of D7 and D7-related proteins was similar in Plasmodium berghei-infected and uninfected mosquitoes.
|Journal or Publication Title:||Insect Molecular Biology|
|Uncontrolled Keywords:||Anopheles stephensi ; salivary glands ; mosquito ; Malaria|
|Subjects:||Q Science > QR Microbiology > QR355 Virology|
|Departments:||Faculty of Health and Medicine > Biomedical & Life Sciences|
|Deposited On:||27 Jul 2012 14:59|
|Last Modified:||12 Mar 2016 01:30|
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