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Combining immunolabeling and surface-enhanced Raman spectroscopy on cell membranes

Hodges, Matt and Kerns, Jemma and Bentley, Adam J. and Fogarty, Simon and Patel, Imran I. and Martin, Francis L. and Fullwood, Nigel J. (2011) Combining immunolabeling and surface-enhanced Raman spectroscopy on cell membranes. ACS Nano, 5 (12). pp. 9535-9541. ISSN 1936-0851

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Abstract

We applied surface-enhanced Raman spectroscopy (SERS) to immunolabeled endothelial cells to derive enhanced spectra of the biomolecular makeup of the cellular surface. A two-step immunolabeling protocol with gold-conjugated antibodies coupled with silver enhancement to attach silver nanoparticles to the cell surface was employed. This approach generated ∼50-fold SERS enhancement of spectral signals. The SERS spectra exhibited several SERS-enhanced peaks associated with cell membrane components. The SERS detection of silver nanoparticles proved more far more sensitive than conventional light microscopy techniques. The SERS enhancement allowed us to carry out spectral mapping using wavenumbers associated with membrane components that correlated directly with the distribution of silver nanoparticles. SERS has the potential to detect immunolabeling at lower levels than is possible using conventional immunolabeling methods while simultaneously providing unique, spatially defined, biochemical information.

Item Type: Article
Journal or Publication Title: ACS Nano
Uncontrolled Keywords: Cell Membrane ; Cells, Cultured ; Epithelium, Corneal ; Epitope Mapping ; Humans ; Immunohistochemistry ; Membrane Proteins ; Spectrum Analysis, Raman
Subjects: UNSPECIFIED
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
Faculty of Health and Medicine > Medicine
Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 56183
Deposited By: ep_importer_pure
Deposited On: 25 Jul 2012 13:37
Refereed?: Yes
Published?: Published
Last Modified: 29 Jul 2014 09:24
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/56183

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