McAinsh, Martin and Ng, Carl K-Y. (2013) Measurement of cytosolic-free Ca2+ in plant tissue. In: Calcium signalling protocols. Methods in Molecular Biology (3rd). Humana Press, USA, pp. 327-341. ISBN 978-1-58829-442-5
Full text not available from this repository.Official URL: http://dx.doi.org/10.1007/978-1-62703-086-1_20
Abstract
A range of techniques have been used to measure the concentration of cytosolic-free Ca2+ ([Ca2+]cyt) in plant cells. Fluorescent Ca2+-sensitive indicators have been used extensively to measure plant [Ca2+]cyt and a number of techniques are available for loading these into plant cells. Here we describe a method for measuring [Ca2+)cyt in the guard cells of the model plant species Commelina communis by ratio photometry and imaging techniques using the ratiometric fluorescent Ca2+-sensitive indicator fura-2
| Item Type: | Contribution in Book/Report/Proceedings |
|---|---|
| Uncontrolled Keywords: | Commelina communis ; guard cell ; fura-2 ; microinjection ; ratiometric measurements |
| Subjects: | Q Science > QK Botany |
| Departments: | Faculty of Science and Technology > Lancaster Environment Centre |
| ID Code: | 56164 |
| Deposited By: | ep_importer_pure |
| Deposited On: | 17 Jul 2012 11:27 |
| Refereed?: | No |
| Published?: | Published |
| Last Modified: | 26 Mar 2013 15:39 |
| Identification Number: | |
| URI: | http://eprints.lancs.ac.uk/id/eprint/56164 |
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