Owen-Lynch, P J and Wong, A K and Whetton, A D (1995) v-Abl-mediated apoptotic suppression is associated with SHC phosphorylation without concomitant mitogen-activated protein kinase activation. The Journal of biological chemistry, 270 (11). pp. 5956-5962. ISSN 0021-9258
Full text not available from this repository.Abstract
A temperature-sensitive mutant of the v-Abl protein has previously been shown to exhibit tyrosine protein kinase activity in Interleukin 3 (IL-3)-dependent IC.DP cells grown at the permissive temperature (32 degrees C) but not at the restrictive temperature (39 degrees C). These IC.DP cells are dependent on IL-3 for suppression of apoptosis at 39 degrees C, but at 32 degrees C cells will survive without added growth factor. Both IL-3 and v-Abl stimulated the tyrosine phosphorylation of SHC and GTPase-activating protein. However, while IL-3 stimulated similar levels of tyrosine phosphorylation in p46shc and p52shc, v-Abl preferentially phosphorylated p52shc, an event that occurred within 1 h of temperature switch. v-Abl also differentially associated with p46shc in a temperature-independent manner. In contrast, only IL-3 stimulated detectable increases in both myelin basic protein kinase and mitogen-activated protein (MAP) kinase kinase in in vitro assays, although in more specific MAP kinase activity assays a very slight increase in the activity of this enzyme was observed after 6 h at the permissive temperature. Time course studies suggest that phosphorylation and association of SHC with v-Abl is insufficient to lead to significant activation of MAP kinase and that activation of the MAP kinase kinase/MAP kinase pathway is not required for apoptotic suppression.
| Item Type: | Article |
|---|---|
| Journal or Publication Title: | The Journal of biological chemistry |
| Subjects: | Q Science > QR Microbiology |
| Departments: | Faculty of Health and Medicine > Biomedical & Life Sciences |
| ID Code: | 56086 |
| Deposited By: | ep_importer_pure |
| Deposited On: | 24 Jul 2012 11:41 |
| Refereed?: | Yes |
| Published?: | Published |
| Last Modified: | 26 Jul 2012 20:46 |
| Identification Number: | |
| URI: | http://eprints.lancs.ac.uk/id/eprint/56086 |
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