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v-Abl-mediated apoptotic suppression is associated with SHC phosphorylation without concomitant mitogen-activated protein kinase activation

Owen-Lynch, P J and Wong, A K and Whetton, A D (1995) v-Abl-mediated apoptotic suppression is associated with SHC phosphorylation without concomitant mitogen-activated protein kinase activation. Journal of Biological Chemistry, 270 (11). pp. 5956-5962. ISSN 0021-9258

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Abstract

A temperature-sensitive mutant of the v-Abl protein has previously been shown to exhibit tyrosine protein kinase activity in Interleukin 3 (IL-3)-dependent IC.DP cells grown at the permissive temperature (32 degrees C) but not at the restrictive temperature (39 degrees C). These IC.DP cells are dependent on IL-3 for suppression of apoptosis at 39 degrees C, but at 32 degrees C cells will survive without added growth factor. Both IL-3 and v-Abl stimulated the tyrosine phosphorylation of SHC and GTPase-activating protein. However, while IL-3 stimulated similar levels of tyrosine phosphorylation in p46shc and p52shc, v-Abl preferentially phosphorylated p52shc, an event that occurred within 1 h of temperature switch. v-Abl also differentially associated with p46shc in a temperature-independent manner. In contrast, only IL-3 stimulated detectable increases in both myelin basic protein kinase and mitogen-activated protein (MAP) kinase kinase in in vitro assays, although in more specific MAP kinase activity assays a very slight increase in the activity of this enzyme was observed after 6 h at the permissive temperature. Time course studies suggest that phosphorylation and association of SHC with v-Abl is insufficient to lead to significant activation of MAP kinase and that activation of the MAP kinase kinase/MAP kinase pathway is not required for apoptotic suppression.

Item Type: Article
Journal or Publication Title: Journal of Biological Chemistry
Subjects: Q Science > QR Microbiology
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 56086
Deposited By: ep_importer_pure
Deposited On: 24 Jul 2012 11:41
Refereed?: Yes
Published?: Published
Last Modified: 01 Oct 2013 11:06
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/56086

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