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Leishmania donovani:immunochemical localization and secretory mechanism of soluble acid phosphatase

Bates, P A and Hermes, I and Dwyer, D M (1989) Leishmania donovani:immunochemical localization and secretory mechanism of soluble acid phosphatase. Experimental Parasitology, 68 (3). pp. 335-346. ISSN 0014-4894

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Abstract

Monoclonal antibodies specific for the soluble, secreted acid phosphatase (EC 3.1.3.2) of Leishmania donovani were used to investigate the localization of this enzyme in extracellular promastigotes and intracellular amastigotes. Indirect immunofluorescence showed a weak general staining in the promastigote cytoplasm, together with strong fluorescence in the flagellar reservoir. Immunofluorescence studies on U937 cells infected in vitro with L. donovani showed that the pathogenic amastigote stage also produced soluble acid phosphatase. Metabolic labeling experiments using promastigotes indicated that the intracellular enzyme was soluble prior to secretion and no evidence was found for the association of secretory acid phosphatase with cell membranes after protein synthesis. The rapid release of acid phosphatase from the flagellar reservoir was energy dependent and may be coupled to beating of the flagellum. The results demonstrated that acid phosphatase was secreted into the flagellar reservoir by Leishmania promastigotes using a conventional constitutive secretory mechanism, and subsequently released from the reservoir into the extracellular medium.

Item Type: Article
Journal or Publication Title: Experimental Parasitology
Uncontrolled Keywords: Leishmania donovani ; Protozoa ; parasitic ; Hemoflagellate ; Acid phosphatase (EC 3.1.3.2) ; Flagellar reservoir ; Secretion ; Promastigote ; Amastigote
Subjects: Q Science > QR Microbiology > QR355 Virology
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 56068
Deposited By: ep_importer_pure
Deposited On: 24 Jul 2012 10:15
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 20:46
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/56068

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