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Axenic cultivation and characterization of Leishmania mexicana amastigote-like forms

Bates, P A and Robertson, C D and Tetley, L and Coombs, G H (1992) Axenic cultivation and characterization of Leishmania mexicana amastigote-like forms. Parasitology, 105 (2). pp. 193-202. ISSN 0031-1820

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    Abstract

    A new method is described which has made possible the long-term axenic cultivation of Leishmania mexicana amastigote-like forms in Schneider's Drosophila medium supplemented with 20% (v/v) foetal calf serum. Unlike previous methods, it utilizes direct culture of parasites obtained from the lesions of infected animals rather than adaptation of promastigotes in vitro. Ultrastructural (possession of megasomes), biochemical (cysteine proteinase activity and gelatin SDS-PAGE banding pattern) and infectivity (in vivo) data are presented which show the close similarity of the cultured forms to lesion amastigotes. The axenically cultured forms grew optimally at a temperature of 32-33 degrees C, providing further evidence for their amastigote nature. It was found that adjustment of the pH of the growth medium to 5.4 was required in order to retain the amastigote morphology of the cultured parasites. This supports the notion that leishmanial amastigotes are acidophiles.

    Item Type: Article
    Journal or Publication Title: Parasitology
    Additional Information: http://journals.cambridge.org/action/displayJournal?jid=PAR The final, definitive version of this article has been published in the Journal, Parasitology, 105 (2), pp 193-202 1992, © 1992 Cambridge University Press.
    Uncontrolled Keywords: Leishmania mexicana ; axenic culture ; amastigotes ; megasomes ; cysteine proteinase ; infectivity
    Subjects: Q Science > QR Microbiology > QR355 Virology
    Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
    ID Code: 56063
    Deposited By: ep_importer_pure
    Deposited On: 24 Jul 2012 09:50
    Refereed?: Yes
    Published?: Published
    Last Modified: 26 Jul 2012 20:46
    Identification Number:
    URI: http://eprints.lancs.ac.uk/id/eprint/56063

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