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Expression of cysteine proteinases by metacyclic promastigotes of Leishmania mexicana

Bates, P A and Robertson, C D and Coombs, G H (1994) Expression of cysteine proteinases by metacyclic promastigotes of Leishmania mexicana. The Journal of eukaryotic microbiology, 41 (3). pp. 199-203. ISSN 1066-5234

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Abstract

The expression of cysteine proteinases by metacyclic promastigotes of Leishmania mexicana was investigated using gelatin polyacrylamide gel electrophoresis. Two prominent bands were detected which distinguished metacyclics from multiplicative promastigotes, lacking detectable cysteine proteinase activity, and amastigotes, with a distinct banding pattern composed of multiple enzymes. A correlation between relative activity of the metacyclic-specific bands and the prevalence of metacyclics was found both during the growth cycle in vitro as metacyclogenesis occurred, and by comparison of stationary phase populations from consecutive subpassages in vitro. Irreversible inhibition of the metacyclic activities using N-benzyloxycarbonyl-phenylalanyl-alanyl diazomethane did not inhibit metacyclic to amastigote transformation in vitro. These activities provide a useful biochemical marker for the metacyclic promastigotes of L. mexicana.

Item Type: Article
Journal or Publication Title: The Journal of eukaryotic microbiology
Uncontrolled Keywords: Amastigote ; metacyclogenesis ; transformation
Subjects: Q Science > QR Microbiology > QR355 Virology
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 56059
Deposited By: ep_importer_pure
Deposited On: 24 Jul 2012 09:36
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 20:46
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/56059

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