Bates, P A and Robertson, C D and Coombs, G H (1994) Expression of cysteine proteinases by metacyclic promastigotes of Leishmania mexicana. Journal of Eukaryotic Microbiology, 41 (3). pp. 199-203. ISSN 1066-5234Full text not available from this repository.
The expression of cysteine proteinases by metacyclic promastigotes of Leishmania mexicana was investigated using gelatin polyacrylamide gel electrophoresis. Two prominent bands were detected which distinguished metacyclics from multiplicative promastigotes, lacking detectable cysteine proteinase activity, and amastigotes, with a distinct banding pattern composed of multiple enzymes. A correlation between relative activity of the metacyclic-specific bands and the prevalence of metacyclics was found both during the growth cycle in vitro as metacyclogenesis occurred, and by comparison of stationary phase populations from consecutive subpassages in vitro. Irreversible inhibition of the metacyclic activities using N-benzyloxycarbonyl-phenylalanyl-alanyl diazomethane did not inhibit metacyclic to amastigote transformation in vitro. These activities provide a useful biochemical marker for the metacyclic promastigotes of L. mexicana.
|Journal or Publication Title:||Journal of Eukaryotic Microbiology|
|Uncontrolled Keywords:||Amastigote ; metacyclogenesis ; transformation|
|Subjects:||Q Science > QR Microbiology > QR355 Virology|
|Departments:||Faculty of Health and Medicine > Biomedical & Life Sciences|
|Deposited On:||24 Jul 2012 09:36|
|Last Modified:||03 Nov 2015 16:10|
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