Lancaster EPrints

In vitro stimulation of metacyclogenesis in Leishmania braziliensis, L. donovani, L. major and L. mexicana

Zakai, H A and Chance, M L and Bates, P A (1998) In vitro stimulation of metacyclogenesis in Leishmania braziliensis, L. donovani, L. major and L. mexicana. Parasitology, 116 (4). pp. 305-309. ISSN 0031-1820

[img]
Preview
PDF - Published Version
Download (241Kb) | Preview

    Abstract

    Promastigotes of Leishmania braziliensis, L. donovani, L. major and L. mexicana recently derived from tissue amastigotes were cultured in Schneider's Drosophila medium supplemented with 20% (v/v) heat-inactivated foetal calf serum and 25 micrograms gentamicin sulfate/ml at pH 5.5. These cultures produced more metacyclic promastigotes in their stationary-phase populations than others cultured at pH 7.0. Metacyclic promastigotes possessed a short (<or = 8 microns) and narrow (<or = 1.5 microns) cell body with a flagellum twice or more the length of the cell body. Promastigotes from acidic cultures were more resistant to complement-mediated lysis and more infective in vivo than those grown at neutral pH. These results demonstrate that induction of metacyclogenesis by acidic pH is a response conserved across a variety of species of Leishmania.

    Item Type: Article
    Journal or Publication Title: Parasitology
    Additional Information: http://journals.cambridge.org/action/displayJournal?jid=PAR The final, definitive version of this article has been published in the Journal, Parasitology, 116 (4), pp 305-309 1998, © 1998 Cambridge University Press.
    Uncontrolled Keywords: Leishmania spp ; promastigote ; metacyclic ; culture ; infectivity ; differentiation
    Subjects: Q Science > QR Microbiology > QR355 Virology
    Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
    ID Code: 56051
    Deposited By: ep_importer_pure
    Deposited On: 20 Jul 2012 14:32
    Refereed?: Yes
    Published?: Published
    Last Modified: 26 Jul 2012 20:46
    Identification Number:
    URI: http://eprints.lancs.ac.uk/id/eprint/56051

    Actions (login required)

    View Item