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Towards a standard battery of microsatellite markers for the analysis of the Leishmania donovani complex

Jamjoom, M B and Ashford, R W and Bates, P A and Kemp, S J and Noyes, H A (2002) Towards a standard battery of microsatellite markers for the analysis of the Leishmania donovani complex. Annals of Tropical Medicine and Parasitology, 96 (3). pp. 265-270. ISSN 0003-4983

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Abstract

The investigation of microsatellite markers has recently superseded that of isoenzymes for many population-biology applications. Microsatellites have the advantages of being dominant, neutral, highly polymorphic and easily scored by high-throughput methods. However, it is necessary to develop a new panel of markers for each group of organisms of interest. Previously, only about 5% of the markers that amplify Leishmania major microsatellite loci were also found to amplify L. donovani loci. A panel of 20 microsatellite markers that are polymorphic in L. donovani and L. infantum has now been developed, using a rapid-enrichment method that will be suitable for developing libraries of markers for other trypanosomatid species. This is the first panel of polymorphic microsatellite markers, to be isolated de novo from any species of Leishmania, that is large enough for population-biology applications.

Item Type: Article
Journal or Publication Title: Annals of Tropical Medicine and Parasitology
Subjects: Q Science > QR Microbiology > QR355 Virology
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 56048
Deposited By: ep_importer_pure
Deposited On: 20 Jul 2012 13:57
Refereed?: Yes
Published?: Published
Last Modified: 09 Apr 2014 23:50
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/56048

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