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Detailed analysis of an experimental challenge model for Leishmania infantum (JPC strain) in dogs

Poot, Jacqueline and Rogers, Matthew E and Bates, Paul A and Vermeulen, Arno (2005) Detailed analysis of an experimental challenge model for Leishmania infantum (JPC strain) in dogs. Veterinary parasitology, 130 (1-2). pp. 41-53. ISSN 0304-4017

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Abstract

In this study, disease progression after intravenous or subdermal infection of dogs with Leishmania infantum JPC strain was monitored. A challenge performed on 14 dogs via the intravenous route with 5 x 10(7) stationary phase promastigotes of the L. infantum JPC strain was 100% successful. During a follow up period of 1.5 years, several parameters were evaluated in order to find the most reliable disease markers. Parasite detection by culture and histology were found to be very sensitive (100%). Additionally, regular physical examination, serology and serum gamma-globulin levels were found to be useful parameters in the evaluation of disease severity and are recommended for inclusion in vaccination-challenge experiments. Although this intravenous challenge model has practical limitations, the data set confirms it is the best experimental model currently available for vaccine development. Two intravenously infected dogs were treated with corticosteroids for 5 months. This treatment was shown to enhance all aspects of a Leishmania infection. Five more dogs were infected by sub-dermal injection of promastigotes mixed with a proteophosphoglycan-matrix (PSG) secreted by Leishmania that assists in transmission and infection by sand fly bite. The resulting parasite burdens were low and the animals remained asymptomatic during a 2-year follow up period. However, this procedure did result in infection in 80% of the dogs and is appealing for future development as a natural challenge model in vaccine development.

Item Type: Article
Journal or Publication Title: Veterinary parasitology
Uncontrolled Keywords: Leishmania infantum ; Dog ; Experimental infection ; Immune response ; PSG
Subjects: Q Science > QR Microbiology > QR355 Virology
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 56044
Deposited By: ep_importer_pure
Deposited On: 20 Jul 2012 13:37
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 20:46
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/56044

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