Bernard, Stéphanie M and Møller, Anders Laurell Blom and Dionisio, Giuseppe and Kichey, Thomas and Jahn, Thomas P and Dubois, Frederic and Baudo, Marcela and Lopes, Marta S and Tercé-Laforgue, Thérèse and Foyer, Christine H and Parry, Martin A J and Forde, Brian G and Araus, Jose Luis and Hirel, Bertrand and Schjoerring, Jan K and Habash, Dimah Z (2008) Gene expression, cellular localisation and function of glutamine synthetase isozymes in wheat (Triticum aestivum L.). Plant Molecular Biology, 67 (1-2). pp. 89-105. ISSN 0167-4412Full text not available from this repository.
We present the first cloning and study of glutamine synthetase (GS) genes in wheat (Triticum aestivum L.). Based on sequence analysis, phylogenetic studies and mapping data, ten GS sequences were classified into four sub-families: GS2 (a, b and c), GS1 (a, b and c), GSr (1 and 2) and GSe (1 and 2). Phylogenetic analysis showed that the wheat GS sub-families together with the GS genes from other monocotyledonous species form four distinct clades. Immunolocalisation studies in leaves, stems and rachis in plants at flowering showed GS protein to be present in parenchyma, phloem companion and perifascicular sheath cells. In situ localisation confirmed that GS1 transcripts were present in the perifascicular sheath cells whilst those for GSr were confined to the vascular cells. Studies of the expression and protein profiles showed that all GS sub-families were differentially expressed in the leaves, peduncle, glumes and roots. Expression of GS genes in leaves was developmentally regulated, with both GS2 and GS1 assimilating or recycling ammonia in leaves during the period of grain development and filling. During leaf senescence the cytosolic isozymes, GS1 and GSr, were the predominant forms, suggesting major roles in assimilating ammonia during the critical phases of remobilisation of nitrogen to the grain. A preliminary analysis of three different wheat genotypes showed that the ratio of leaf GS2 protein to GS1 protein was variable. Use of this genetic variation should inform future efforts to modulate this enzyme for pre-breeding efforts to improve nitrogen use in wheat.
|Journal or Publication Title:||Plant Molecular Biology|
|Uncontrolled Keywords:||Glutamine synthetase ; Wheat ; Senescence ; Nitrogen ; Gene expression ; In situ hybridisation ; Immunolocalisation|
|Subjects:||G Geography. Anthropology. Recreation > GE Environmental Sciences|
|Departments:||Faculty of Science and Technology > Lancaster Environment Centre|
|Deposited On:||12 Jul 2012 16:17|
|Last Modified:||12 Sep 2015 01:04|
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