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Accumulation, subcellular distribution and toxicity of copper in earthworm (Eisenia fetida) in the presence of ciprofloxacin

Huang, Rixiang and Wen, Bei and Pei, Zhiguo and Shan, Xiao-Quan and Zhang, Shuzhen and Williams, Paul N (2009) Accumulation, subcellular distribution and toxicity of copper in earthworm (Eisenia fetida) in the presence of ciprofloxacin. Environmental Science and Technology, 43 (10). pp. 3688-3693. ISSN 0013-936X

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Abstract

Land application of wastes from concentrated animal feeding operations results in accumulation of copper (Cu) and antimicrobials in terrestrial systems. Interaction between Cu and antimicrobials may change Cu speciation in soil solution, and affect Cu bioavailability and toxicity. In this study, earthworms were exposed to quartz sand percolated with different concentrations of Cu and ciprofloxacin (CIP). Copper uptake by earthworms, its subcellular partition, and toxicity were studied. An increase in the applied CIP decreased the free Cu ion concentration in external solution and mortalities of earthworm, while Cu contents in earthworms increased. Copper and CIP in earthworms were fractionated into five fractions: a granular fraction (D), a fraction consisting of tissue fragments, cell membranes, and intact cells (E), a microsomal fraction (F), a denatured proteins fraction (G), and a heat-stable proteins fraction (H). Most of the CIP in earthworms was in fraction H. Copper was redistributed from the metal-sensitive fraction E to fractions D, F, G, and H with increasing CIP concentration. These results challenge the free ion activity model and suggested that Cu may be partly taken up as Cu-CIP complexes in earthworms, changing the bioavailability, subcellular distribution, and toxicity of Cu to earthworms.

Item Type: Article
Journal or Publication Title: Environmental Science and Technology
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 53855
Deposited By: ep_importer_pure
Deposited On: 27 Apr 2012 16:18
Refereed?: Yes
Published?: Published
Last Modified: 09 Apr 2014 23:23
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/53855

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