Smallwood, M and Worrall, D and Byass, L and Elias, L and Ashford, D and Doucet, C J and Holt, C and Telford, J and Lillford, P and Bowles, D J (1999) Isolation and characterization of a novel antifreeze protein from carrot (Daucus carota). Biochemical Journal, 340 (2). pp. 385-391. ISSN 0264-6021
Full text not available from this repository.Abstract
A modified assay for inhibition of ice recrystallization which allows unequivocal identification of activity in plant extracts is described. Using this assay a novel, cold-induced, 36 kDa antifreeze protein has been isolated from the tap root of cold-acclimated carrot (Daucus carota) plants, This protein inhibits the recrystallization of ice and exhibits thermal-hysteresis activity. The polypeptide behaves as monomer in solution and is N- glycosylated, The corresponding gene is unique in the carrot genome and induced by cold, The antifreeze protein appears to be localized within the apoplast.
| Item Type: | Article |
|---|---|
| Journal or Publication Title: | Biochemical Journal |
| Uncontrolled Keywords: | apoplast ; cold-on-regulated gene ; ice recrystallization inhibition ; polygalacturonase inhibitor protein ; thermal hysteresis ; THERMAL HYSTERESIS PROTEIN ; N-LINKED OLIGOSACCHARIDES ; ICE RECRYSTALLIZATION ; WINTER RYE ; PLANT ; SEQUENCE |
| Subjects: | UNSPECIFIED |
| Departments: | Faculty of Science and Technology > Lancaster Environment Centre |
| ID Code: | 53552 |
| Deposited By: | ep_importer_pure |
| Deposited On: | 19 Apr 2012 10:03 |
| Refereed?: | Yes |
| Published?: | Published |
| Last Modified: | 26 Jul 2012 20:16 |
| Identification Number: | |
| URI: | http://eprints.lancs.ac.uk/id/eprint/53552 |
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