Lancaster EPrints

Isolation and characterization of a novel antifreeze protein from carrot (Daucus carota)

Smallwood, M and Worrall, D and Byass, L and Elias, L and Ashford, D and Doucet, C J and Holt, C and Telford, J and Lillford, P and Bowles, D J (1999) Isolation and characterization of a novel antifreeze protein from carrot (Daucus carota). Biochemical Journal, 340 (2). pp. 385-391. ISSN 0264-6021

Full text not available from this repository.

Abstract

A modified assay for inhibition of ice recrystallization which allows unequivocal identification of activity in plant extracts is described. Using this assay a novel, cold-induced, 36 kDa antifreeze protein has been isolated from the tap root of cold-acclimated carrot (Daucus carota) plants, This protein inhibits the recrystallization of ice and exhibits thermal-hysteresis activity. The polypeptide behaves as monomer in solution and is N- glycosylated, The corresponding gene is unique in the carrot genome and induced by cold, The antifreeze protein appears to be localized within the apoplast.

Item Type: Article
Journal or Publication Title: Biochemical Journal
Uncontrolled Keywords: apoplast ; cold-on-regulated gene ; ice recrystallization inhibition ; polygalacturonase inhibitor protein ; thermal hysteresis ; THERMAL HYSTERESIS PROTEIN ; N-LINKED OLIGOSACCHARIDES ; ICE RECRYSTALLIZATION ; WINTER RYE ; PLANT ; SEQUENCE
Subjects: UNSPECIFIED
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 53552
Deposited By: ep_importer_pure
Deposited On: 19 Apr 2012 10:03
Refereed?: Yes
Published?: Published
Last Modified: 09 Apr 2014 23:19
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/53552

Actions (login required)

View Item