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Excision of the maize transposable element Ac in flax callus

Roberts, Michael and Kumar, Amar and Scott, Rod and Draper, John (1990) Excision of the maize transposable element Ac in flax callus. Plant Cell Reports, 9 (7). pp. 406-409. ISSN 0721-7714

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Abstract

The frequency and fidelity of Ac transposition, and that of its non-autonomous derivative Ds, were investigated in flax callus. Flax (Linum usitatissimum var. Antares) hypocotyls were transformed with Agrobacterium Ti plasmid vectors containing the Ac or Ds element inserted within the untranslated leader sequence of a chimaeric neomycin phosphotransferase II gene. Kanamycin resistant tissues were produced as a result of excision of Ac in around 35% of the total number of Ac-containing transformants. In contrast, no excision was observed from transformants containing the Ds element. Whilst Ac appears to have excised completely from T-DNAs, little evidence was found to infer reintegration of the Ac element into the genome.

Item Type: Article
Journal or Publication Title: Plant Cell Reports
Uncontrolled Keywords: flax ; transposon
Subjects:
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 53075
Deposited By: ep_importer_pure
Deposited On: 09 Mar 2012 02:44
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 19:33
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/53075

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