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Isolation of a mRNA encoding a nucleoside diphosphate kinase from tomato that is up-regulated by wounding

Harris, Neil and Taylor, Jane E and Roberts, Jeremy A (1994) Isolation of a mRNA encoding a nucleoside diphosphate kinase from tomato that is up-regulated by wounding. Plant Molecular Biology, 25 (4). pp. 739-742. ISSN 0167-4412

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Abstract

A cDNA clone (TAB2) encoding a nucleoside diphosphate (NDP) kinase has been isolated from a tomato (Lycopersicon esculentum Mill. cv. Ailsa Craig) cDNA library. The clone is 590 bp long and exhibits a high degree of sequence identity with spinach NDP kinases I and II, Pisum sativum NDP kinase I, Arabidopsis thaliana NDP kinase, Drosophila melanogaster NDP kinase, Dictyostelium discoideum NDP kinase and human Nm 23-H1 and Nm23-H2. Northern analysis has revealed that the mRNA encoded by TAB2 is up-regulated in both leaf and stem tissue in response to wounding. The increase is apparent within 1 h of wounding and is not further elevated by application of ethylene. Southern blot analysis indicates that TAB2 is a member of a small gene family.

Item Type: Article
Journal or Publication Title: Plant Molecular Biology
Uncontrolled Keywords: Lycopersicon esculentum ; tomato ; nucleoside diphosphate kinase ; wounding ; ethylene
Subjects: UNSPECIFIED
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 52484
Deposited By: ep_importer_pure
Deposited On: 08 Feb 2012 12:02
Refereed?: Yes
Published?: Published
Last Modified: 09 Apr 2014 23:04
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/52484

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