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Characterisation and accumulation pattern of a mRNA encoding an abscission-related β-1, 4-glucanase from Sambucus nigra

Taylor, Jane E and Coupe, Simon A. and Picton, Steve J and Roberts, Jeremy A (1994) Characterisation and accumulation pattern of a mRNA encoding an abscission-related β-1, 4-glucanase from Sambucus nigra. Plant Molecular Biology, 24 (6). pp. 961-964. ISSN 0167-4412

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Abstract

A cDNA library was produced using mRNA extracted from ethylene-treated leaflet abscission zones of common elder (Sambucus nigra). Screening of the library with the insert from pBAC10, which encodes an abscission -1,4-glucanase (cellulase) from bean (Phaseolus vulgaris), resulted in the isolation of a near-full-length cDNA which was designated JET 1. Northern analysis, using JET 1 as a probe, detected a transcript of 1.9 kb that accumulated prior to the first visible signs of cell separation. Accumulation of the JET 1 transcript is promoted by ethylene and primarily restricted to the tissue comprising the abscission zone. Sequence analysis of JET 1 indicates it is 1768 bp in length and shares significant homology at the amino acid level with -1,4-glucanases from the leaf abscission zone of P. vulgaris (67%) and ripening avocado fruit (48%). The predicted peptide sequence of the S. nigra enzyme contains two potential glycosylation sites. Genomic Southern analysis of S. nigra DNA reveals that JET 1 may belong to a multi-gene family.

Item Type: Article
Journal or Publication Title: Plant Molecular Biology
Uncontrolled Keywords: abscission ; -1,4-glucanase ; cellulase ; ethylene ; Sambucus nigra
Subjects:
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 52483
Deposited By: ep_importer_pure
Deposited On: 08 Feb 2012 11:59
Refereed?: Yes
Published?: Published
Last Modified: 09 Apr 2014 23:04
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/52483

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