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Purification of β-1, 4-glucanase from ethylene-treated abscission zones of Sambucus nigra

Webb, Simon TJ and Taylor, Jane E and Coupe, Simon A and Tucker, Gregory A and Ferrarese, L. and Roberts, Jeremy A (1993) Purification of β-1, 4-glucanase from ethylene-treated abscission zones of Sambucus nigra. Plant, Cell and Environment, 16 (3). pp. 329-333. ISSN 0140-7791

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Abstract

Leaflet abscission in Sambucus nigra is the result of cell wall breakdown at the site of separation. Associated with wall degradation is an increase in the activity of the enzyme β1,4 glucanase (E.C.3.1.2.4) in the cells that comprise the abscission zone. The enzyme has been extracted from abscission zone tissue and purified using a substrate affinity column. A qualitative enzyme assay procedure has been developed and this has facilitated the purification process. The β1,4 glucanase enzyme has a pH optimum of 7 and a molecular mass of 54kDa. Antibodies have been raised to the purified protein. The role of the enzyme in the abscission process is discussed.

Item Type: Article
Journal or Publication Title: Plant, Cell and Environment
Uncontrolled Keywords: Sambucus nigra ; elder ; abscission zone ; cell separation ; β1,4 glucanase ; ethylene ; antibody
Subjects:
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 52479
Deposited By: ep_importer_pure
Deposited On: 08 Feb 2012 10:22
Refereed?: Yes
Published?: Published
Last Modified: 09 Apr 2014 23:04
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/52479

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