Lancaster EPrints

Changes in polygalacturonase activity and solubility of polyuronides during ethylene-stimulated leaf abscission in Sambucus nigra

Taylor, Jane E and Webb, Simon TJ and Coupe, Simon A and Tucker, Gregory A and Roberts, Jeremy A (1993) Changes in polygalacturonase activity and solubility of polyuronides during ethylene-stimulated leaf abscission in Sambucus nigra. Journal of Experimental Botany, 44 (1). pp. 93-98. ISSN 1460-2431

Full text not available from this repository.

Abstract

Leaflet abscission in Sambucus nigra is precipitated by cell wall degradation which is restricted to the site of cell separation. Accompanying wall breakdown is an increase in the activity of the enzyme polygalacturonase (PG) (E.C. 3.2.1.15) and this rise is primarily confined to the abscission zone tissue. The polygalacturonase has a pH optimum of 4·4 and has the characteristics of an endo-acting enzyme. The elevation in enzyme activity is the result of an increase in at least two isoforms of PG as revealed by polyacrylamide gel electrophoresis of the natured protein. Leaflet abscission in S. nigra is associated with an increase in the solubility and depolymerization of polyuronides from the cell wall. These observations are discussed in relation to the mechanism of cell separation during ethylene-stimulated leaf abscission.

Item Type: Article
Journal or Publication Title: Journal of Experimental Botany
Uncontrolled Keywords: Elder ; Sambucus nigra ; abscission ; polygalacturonase ; polyuronides ; ethylene
Subjects: UNSPECIFIED
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 52476
Deposited By: ep_importer_pure
Deposited On: 08 Feb 2012 09:59
Refereed?: Yes
Published?: Published
Last Modified: 09 Apr 2014 23:03
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/52476

Actions (login required)

View Item