Taylor, Jane E and Webb, Simon TJ and Coupe, Simon A and Tucker, Gregory A and Roberts, Jeremy A (1993) Changes in polygalacturonase activity and solubility of polyuronides during ethylene-stimulated leaf abscission in Sambucus nigra. Journal of Experimental Botany, 44 (1). pp. 93-98. ISSN 1460-2431Full text not available from this repository.
Leaflet abscission in Sambucus nigra is precipitated by cell wall degradation which is restricted to the site of cell separation. Accompanying wall breakdown is an increase in the activity of the enzyme polygalacturonase (PG) (E.C. 18.104.22.168) and this rise is primarily confined to the abscission zone tissue. The polygalacturonase has a pH optimum of 4·4 and has the characteristics of an endo-acting enzyme. The elevation in enzyme activity is the result of an increase in at least two isoforms of PG as revealed by polyacrylamide gel electrophoresis of the natured protein. Leaflet abscission in S. nigra is associated with an increase in the solubility and depolymerization of polyuronides from the cell wall. These observations are discussed in relation to the mechanism of cell separation during ethylene-stimulated leaf abscission.
|Journal or Publication Title:||Journal of Experimental Botany|
|Uncontrolled Keywords:||Elder ; Sambucus nigra ; abscission ; polygalacturonase ; polyuronides ; ethylene|
|Departments:||Faculty of Science and Technology > Lancaster Environment Centre|
|Deposited On:||08 Feb 2012 09:59|
|Last Modified:||25 Oct 2016 02:27|
Actions (login required)