Munkley, Jennifer and Copeland, Nikki A and Moignard, Victoria and Knight, John R P and Greaves, Erin and Ramsbottom, Simon A and Pownall, Mary E and Southgate, Jennifer and Ainscough, Justin F-X and Coverley, Dawn (2011) Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells. Nucleic Acids Research, 39 (7). pp. 2671-2677. ISSN 0305-1048Full text not available from this repository.
Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In murine embryonic stem cells, Xenopus embryos and human urothelial cells, cyclin E is recruited to the nuclear matrix as cells differentiate and this can be manipulated in vitro. This suggests that pre-RC assembly becomes spatially restricted as template usage is defined. Furthermore, failure to become restricted may contribute to the plasticity of cancer cells.
|Journal or Publication Title:||Nucleic Acids Research|
|Uncontrolled Keywords:||Active Transport, Cell Nucleus ; Animals ; Cell Differentiation ; Cell Line, Tumor ; Cells, Cultured ; Cyclin E ; Humans ; Mice ; Neoplasms ; Nuclear Matrix ; Protein Transport ; Xenopus laevis|
|Departments:||Faculty of Health and Medicine > Biomedical & Life Sciences|
|Deposited On:||27 Jan 2012 12:31|
|Last Modified:||07 Jan 2015 17:11|
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