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Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells

Munkley, Jennifer and Copeland, Nikki A and Moignard, Victoria and Knight, John R P and Greaves, Erin and Ramsbottom, Simon A and Pownall, Mary E and Southgate, Jennifer and Ainscough, Justin F-X and Coverley, Dawn (2011) Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells. Nucleic Acids Research, 39 (7). pp. 2671-2677. ISSN 0305-1048

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Abstract

Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In murine embryonic stem cells, Xenopus embryos and human urothelial cells, cyclin E is recruited to the nuclear matrix as cells differentiate and this can be manipulated in vitro. This suggests that pre-RC assembly becomes spatially restricted as template usage is defined. Furthermore, failure to become restricted may contribute to the plasticity of cancer cells.

Item Type: Article
Journal or Publication Title: Nucleic Acids Research
Uncontrolled Keywords: Active Transport, Cell Nucleus ; Animals ; Cell Differentiation ; Cell Line, Tumor ; Cells, Cultured ; Cyclin E ; Humans ; Mice ; Neoplasms ; Nuclear Matrix ; Protein Transport ; Xenopus laevis
Subjects: UNSPECIFIED
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 52421
Deposited By: ep_importer_pure
Deposited On: 27 Jan 2012 12:31
Refereed?: Yes
Published?: Published
Last Modified: 01 Oct 2013 11:20
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/52421

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