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A Peptide Encoding a B-cell Epitope from the N-Terminus of the Capsid Protein L2 of Bovine Papillomavirus-4 Prevents Disease

Campo, M. Saveria and O'neil, Brian and Grindlay, G. Joan and Curtis, Fiona and Knowles, Graham and Chandrachud, Lata (1997) A Peptide Encoding a B-cell Epitope from the N-Terminus of the Capsid Protein L2 of Bovine Papillomavirus-4 Prevents Disease. Virology, 234 (2). pp. 261-266.

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Abstract

The first 200 N-terminus amino acids of the L2 capsid protein of BPV-4 (designated L2a) are an effective prophylactic vaccine against BPV-4 infection. Vaccination with L2a induces the production of virus neutralizing antibodies, and when L2a antibodies are removed from immune sera, the sera lose their neutralization activity. L2a encodes three dominant B-cell epitopes, defined as epitope 1 (amino acids 101–120), epitope 2 (aa 131–151), and epitope 3 (aa 151–170). To investigate whether any of these epitopes are responsible individually or in combination for protection against viral challenge, synthetic peptides, corresponding to the three epitopes (peptides 11, 14, and 16, respectively) and conjugated to keyhole limpet hemocyanin (KLH) were tested in vaccination challenge experiments. Calves vaccinated with the three peptides together showed no evidence of papillomavirus infection; those vaccinated with peptide 14 alone developed only early lesions which did not progress to proper papillomas and regressed rapidly; those vaccinated with peptide 11 or peptide 16 alone were not protected and proceeded to develop papillomas. Therefore the three B-cell epitopes are not conventionally “neutralizing” when presented individually, but in combination they form a complex neutralization domain, and, in particular, epitope 2, represented by peptide 14, encodes a domain responsible for disease prevention.

Item Type: Article
Journal or Publication Title: Virology
Subjects: UNSPECIFIED
Departments: Faculty of Health and Medicine > Medicine
ID Code: 51952
Deposited By: ep_importer_pure
Deposited On: 09 Dec 2011 11:57
Refereed?: Yes
Published?: Published
Last Modified: 09 Apr 2014 22:56
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/51952

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