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Functional similarities between phage Lambda Orf and Escherichia coli RecFOR in initiation of genetic exchange

Maxwell, Karen and Reed, Patricia and Zhang, Rong-guang and Beasley, Steven and Walmsley, Adrian and Curtis, Fiona and Joachimiak, Andrej and Edwards, Aled and Sharples, Gary (2005) Functional similarities between phage Lambda Orf and Escherichia coli RecFOR in initiation of genetic exchange. Proceedings of the National Academy of Sciences, 102 (32). pp. 11260-11265. ISSN 0027-8424

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Genetic recombination in bacteriophage relies on DNA end processing by Exo to expose 3'-tailed strands for annealing and exchange by protein. Phage encodes an additional recombinase, Orf, which participates in the early stages of recombination by supplying a function equivalent to the Escherichia coli RecFOR complex. These host enzymes assist loading of the RecA strand exchange protein onto ssDNA coated with ssDNA-binding protein. In this study, we purified the Orf protein, analyzed its biochemical properties, and determined its crystal structure at 2.5 Å. The homodimeric Orf protein is arranged as a toroid with a shallow U-shaped cleft, lined with basic residues, running perpendicular to the central cavity. Orf binds DNA, favoring single-stranded over duplex and with no obvious preference for gapped, 3'-tailed, or 5'-tailed substrates. An interaction between Orf and ssDNA-binding protein was indicated by far Western analysis. The functional similarities between Orf and RecFOR are discussed in relation to the early steps of recombinational exchange and the interplay between phage and bacterial recombinases.

Item Type: Journal Article
Journal or Publication Title: Proceedings of the National Academy of Sciences
Uncontrolled Keywords: /dk/atira/pure/core/keywords/biologicalsciences/biomedicalandlifesciences
Departments: Faculty of Health and Medicine > Medicine
ID Code: 51947
Deposited By: ep_importer_pure
Deposited On: 09 Dec 2011 11:07
Refereed?: Yes
Published?: Published
Last Modified: 29 Apr 2019 15:10
Identification Number:

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