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Isoprene synthesis in plants: lessons from a transgenic tobacco model

Vickers, Claudia E. and Possell, Malcolm and Laothawornkitkul, Jullada and Ryan, Annette and Hewitt, C. N. and Mullineaux, Philip M. (2011) Isoprene synthesis in plants: lessons from a transgenic tobacco model. Plant, Cell and Environment, 34 (6). pp. 1043-1053. ISSN 0140-7791

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Abstract

Isoprene is a highly reactive gas, and is emitted in such large quantities from the biosphere that it substantially affects the oxidizing potential of the atmosphere. Relatively little is known about the control of isoprene emission at the molecular level. Using transgenic tobacco lines harbouring a poplar isoprene synthase gene, we examined control of isoprene emission. Isoprene synthase required chloroplastic localization for catalytic activity, and isoprene was produced via the methyl erythritol (MEP) pathway from recently assimilated carbon. Emission patterns in transgenic tobacco plants were remarkably similar to naturally emitting plants under a wide variety of conditions. Emissions correlated with photosynthetic rates in developing and mature leaves, and with the amount of isoprene synthase protein in mature leaves. Isoprene synthase protein levels did not change under short-term increase in heat/light, despite an increase in emissions under these conditions. A robust circadian pattern could be observed in emissions from long-day plants. The data support the idea that substrate supply and changes in enzyme kinetics (rather than changes in isoprene synthase levels or post-translational regulation of activity) are the primary controls on isoprene emission in mature transgenic tobacco leaves.

Item Type: Article
Journal or Publication Title: Plant, Cell and Environment
Uncontrolled Keywords: chloroplast ; circadian rhythms ; isoprene synthase ; GREY POPLAR LEAVES ; VOLATILE ISOPRENOIDS ; GENE-EXPRESSION ; DIMETHYLALLYL DIPHOSPHATE ; BIOGENIC ISOPRENE ; CIRCADIAN CONTROL ; SYNTHASE ACTIVITY ; EMISSION FACTOR ; QUERCUS-ROBUR ; P-31 NMR
Subjects: UNSPECIFIED
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 51530
Deposited By: ep_importer_pure
Deposited On: 25 Nov 2011 12:29
Refereed?: Yes
Published?: Published
Last Modified: 06 Sep 2013 23:09
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/51530

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