Vickers, Claudia E. and Possell, Malcolm and Laothawornkitkul, Jullada and Ryan, Annette and Hewitt, C. N. and Mullineaux, Philip M. (2011) Isoprene synthesis in plants: lessons from a transgenic tobacco model. Plant, Cell and Environment, 34 (6). pp. 1043-1053. ISSN 0140-7791Full text not available from this repository.
Isoprene is a highly reactive gas, and is emitted in such large quantities from the biosphere that it substantially affects the oxidizing potential of the atmosphere. Relatively little is known about the control of isoprene emission at the molecular level. Using transgenic tobacco lines harbouring a poplar isoprene synthase gene, we examined control of isoprene emission. Isoprene synthase required chloroplastic localization for catalytic activity, and isoprene was produced via the methyl erythritol (MEP) pathway from recently assimilated carbon. Emission patterns in transgenic tobacco plants were remarkably similar to naturally emitting plants under a wide variety of conditions. Emissions correlated with photosynthetic rates in developing and mature leaves, and with the amount of isoprene synthase protein in mature leaves. Isoprene synthase protein levels did not change under short-term increase in heat/light, despite an increase in emissions under these conditions. A robust circadian pattern could be observed in emissions from long-day plants. The data support the idea that substrate supply and changes in enzyme kinetics (rather than changes in isoprene synthase levels or post-translational regulation of activity) are the primary controls on isoprene emission in mature transgenic tobacco leaves.
|Journal or Publication Title:||Plant, Cell and Environment|
|Uncontrolled Keywords:||chloroplast ; circadian rhythms ; isoprene synthase ; GREY POPLAR LEAVES ; VOLATILE ISOPRENOIDS ; GENE-EXPRESSION ; DIMETHYLALLYL DIPHOSPHATE ; BIOGENIC ISOPRENE ; CIRCADIAN CONTROL ; SYNTHASE ACTIVITY ; EMISSION FACTOR ; QUERCUS-ROBUR ; P-31 NMR|
|Departments:||Faculty of Science and Technology > Lancaster Environment Centre|
|Deposited On:||25 Nov 2011 12:29|
|Last Modified:||21 Jan 2017 02:15|
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