Popplewell, Jonathan and Swann, Marcus and Brown, Gavin and Lauder, Bob (2012) Fabrication of carbohydrate surfaces by using non-derivatised oligosaccharides. Methods in molecular biology (Clifton, N.J.), 808. pp. 221-229.
Full text not available from this repository.Abstract
Surface-based tools, such as microarrays and optical biosensors, are being increasingly applied to the analysis of carbohydrate-protein interactions. A key to these developments is the presentation of the carbohydrate to the protein target. Dual polarisation interferometry (DPI) is a surface-based technique that permits the real-time measurement of the changes in thickness, refractive index, and mass of adsorbates 100-nm thick or less on the surface of a functionalised waveguide. DPI has been used to design and characterise a surface on which the orientation and density of the immobilised carbohydrates are suitable for studying their interactions with proteins and where non-specific binding is reduced to less than 5% of total binding. A thiol-functionalised surface was derivatised with a heterobifunctional cross-linker to yield a hydrazide surface. This was treated with oligosaccharides, derived from keratan sulphate, chondroitin sulphate, and heparin that possess a reducing end. To block the unreacted hydrazide groups, the surface was treated with an aldehyde-functionalised PEG, and the surfaces were then challenged with a variety of proteins.
| Item Type: | Article |
|---|---|
| Journal or Publication Title: | Methods in molecular biology (Clifton, N.J.) |
| Uncontrolled Keywords: | Chondroitin ; Keratan |
| Subjects: | UNSPECIFIED |
| Departments: | Faculty of Health and Medicine > Biomedical & Life Sciences |
| ID Code: | 51373 |
| Deposited By: | ep_importer_pure |
| Deposited On: | 22 Nov 2011 16:50 |
| Refereed?: | Yes |
| Published?: | Published |
| Last Modified: | 26 Mar 2013 16:02 |
| Identification Number: | |
| URI: | http://eprints.lancs.ac.uk/id/eprint/51373 |
Actions (login required)
| View Item |
