Rimmer, Stephen and Johnson, Claire and Zhao, Bojun and Collier, Joyleen and Gilmore, Louisa and Sabnis, Subodh and Wyman, Paul and Sammon, Christopher and Fullwood, Nigel J. and MacNeil, Sheila (2007) Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells. Biomaterials, 28 (35). pp. 5319-5331. ISSN 0142-9612Full text not available from this repository.
The aim of this study was to develop a hydrogel which would be suitable for corneal cell re-epithelialization when used as a corneal implant. To achieve this, a series of hydrogels were functionalized with primary amines by post-polymerization reactions between amine compounds and glycidyl ether groups attached to the hydrogels. We report a strong correlation between the structure of the amine and the viability of stromal cells and epithelial cells cultured on these hydrogels. Subsequent co-culture of epithelial and stromal cells on the amine modified hydrogels allowed successful expansion of epithelial cells on surfaces functionalized with alkyl alpha-omega diamines with carbon chain lengths of between 3 and 6. Analysis of variance showed that corneal epithelial cells had a strong preference for surfaces functionalized by the reaction of excess 1,3 diaminopropane with units of glycidyl methacrylate compared to the reaction products of other amines (ammonia; 1,2-diaminoethane; 1,4-diaminobutane or 1,6-diaminohexane). We suggest this approach of amine functionalization combined with stromal/epithelial co-culture offers a promising new approach to achieving a secure corneal epithelium.
|Journal or Publication Title:||Biomaterials|
|Uncontrolled Keywords:||hydrogels ; amines ; cornea ; epithelial cells ; METHACRYLATE-BLOCK-(2,3 PROPANDIOL-1-METHACRYLATE-STAT-ETHANDIOL DIMETHACRYLATE)) ; XENOBIOTIC-FREE CULTURE ; AMPHIPHILIC NETWORKS ; HUMAN KERATINOCYTES ; EXTRACELLULAR-MATRIX ; DERMAL FIBROBLASTS ; BLOCK CONETWORKS ; CHITOSAN FILMS ; CLINICAL-USE ; ADHESION|
|Departments:||Faculty of Health and Medicine > Biomedical & Life Sciences|
|Deposited On:||18 Nov 2011 09:44|
|Last Modified:||18 Jun 2015 05:38|
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