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Mcm10 interacts with Rad4/Cut5(TopBP1) and its association with origins of DNA replication is dependent on Rad4/Cut5(TopBP1)

Taylor, Mark and Moore, Karen and Murray, Johanne and Aves, Stephen J and Price, Clive (2011) Mcm10 interacts with Rad4/Cut5(TopBP1) and its association with origins of DNA replication is dependent on Rad4/Cut5(TopBP1). DNA Repair, 10 (11). pp. 1154-1163.

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Abstract

Initiation of DNA replication in eukaryotes is a highly conserved and ordered process involving the co-ordinated, stepwise association of distinct proteins at multiple origins of replication throughout the genome. Here, taking Schizosaccharomyces pombe as a model, the role of Rad4(TopBP1) in the assembly of the replication complex has been examined. Quantitative chromatin immunoprecipitation experiments confirm that Rad4(TopBP1) associates with origins of DNA replication and, in addition, demonstrate that the protein is not present within the active replisome. A direct interaction between Rad4(TopBP1) and Mcm10 is shown and this is reflected in the Rad4(TopBP1)-dependent origin association of Mcm10. Rad4(TopBP1) is also shown to interact with Sld2 and Sld3 and to be required for the stable origin association of these two proteins. Rad4(TopBP1) chromatin association at stalled replication forks was found to be dependent upon the checkpoint protein Rad9, which was not required for Rad4(TopBP1) origin association. Comparison of the levels of chromatin association at origins of replication and stalled replication forks and the differential requirement for Rad9 suggest functional differences for Rad4(TopBP1) at these distinct sites.

Item Type: Article
Journal or Publication Title: DNA Repair
Additional Information: Copyright © 2011 Elsevier B.V. All rights reserved.
Uncontrolled Keywords: TopBP1 ; Eukaryotic DNA replication ; Checkpoint control
Subjects:
Departments:
ID Code: 50877
Deposited By: ep_importer_pure
Deposited On: 08 Nov 2011 11:53
Refereed?: Yes
Published?: Published
Last Modified: 23 Jul 2014 14:55
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/50877

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