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A demonstration of the advantages of immunostaining in the quantification of amyloid plaque deposits

Gentleman, S M and Bruton, C and Allsop, D and Lewis, S J and Polak, J M and Roberts, G W (1989) A demonstration of the advantages of immunostaining in the quantification of amyloid plaque deposits. Histochemistry, 92 (4). pp. 355-358.

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Abstract

Extracellular amyloid deposits are a feature of both Alzheimer type dementia and the 'normal' aging process. Quantification of amyloid plaque deposits may well be useful in distinguishing between the senescent changes associated with 'normal' aging and the pathological processes underlying dementia. To determine the most reliable and reproducible method for visualisation of the amyloid we have compared conventional silver staining techniques with beta-amyloid immunocytochemistry on a large sample of post-mortem brain tissue from both demented (n = 15, age range 60-87) and non-demented (n = 65, age range 14-99) patients. The degree of amyloid deposition was rated on a four point scale and ratings for the two techniques were significantly correlated (P less than 0.01). However, the immunocytochemical approach has a number of distinct advantages for quantification. The antibody to beta-amyloid is highly specific and does not stain neurofibrillary tangles or background features, it is considerably more sensitive than silver staining in highlighting diffuse amyloid deposits and, perhaps most importantly, it produces high contrast staining which allows easier image digitisation and subsequent computer image analysis.

Item Type: Article
Journal or Publication Title: Histochemistry
Uncontrolled Keywords: Adolescent ; Adult ; Aged ; Aged, 80 and over ; Alzheimer Disease ; Amyloid ; Brain ; Brain Chemistry ; Humans ; Immunohistochemistry ; Middle Aged ; Silver ; Staining and Labeling
Subjects:
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
Faculty of Health and Medicine
Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 50867
Deposited By: ep_importer_pure
Deposited On: 08 Nov 2011 11:42
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 19:44
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/50867

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