Kametani, F and Tanaka, K and Ishii, T and Ikeda, S and Kennedy, H E and Allsop, D (1993) Secretory form of Alzheimer amyloid precursor protein 695 in human brain lacks β/A4 amyloid immunoreactivity. Biochemical and Biophysical Research Communications, 191 (2). pp. 392-398. ISSN 0006-291XFull text not available from this repository.
It is not clear how Alzheimer amyloid precursor proteins (APP) are metabolized in the brain itself. Secretory forms of APP in a phosphate buffer-soluble fraction were purified from post-mortem human brain by heparin-affinity and ion-exchange chromatography and analyzed by N-terminal amino acid sequencing and SDS polyacrylamide gel electrophoresis/immunoblotting. We found apparently similar multi-isoforms of secretory APP (at 93-97, 105-112 and 123 KDa) to those that we have described recently in cerebrospinal fluid. Antisera to the initial part of the beta/A4 sequence labelled only those bands that were found to react with antiserum to the Kunitz-type inhibitor insert of APP, suggesting that beta/A4 amyloid may be generated specifically from APP-695.
|Journal or Publication Title:||Biochemical and Biophysical Research Communications|
|Uncontrolled Keywords:||Adult ; Aged ; Alzheimer Disease ; Amino Acid Sequence ; Amyloid ; Amyloid beta-Protein Precursor ; Brain ; Down Syndrome ; Female ; Humans ; Male ; Middle Aged ; Molecular Sequence Data|
|Departments:||Faculty of Health and Medicine > Biomedical & Life Sciences|
|Deposited On:||08 Nov 2011 10:17|
|Last Modified:||09 Feb 2016 01:10|
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