Kametani, F and Tanaka, K and Ishii, T and Ikeda, S and Kennedy, H E and Allsop, D (1993) Secretory form of Alzheimer amyloid precursor protein 695 in human brain lacks β/A4 amyloid immunoreactivity. Biochemical and Biophysical Research Communications, 191 (2). pp. 392-398. ISSN 0006-291X
Full text not available from this repository.Abstract
It is not clear how Alzheimer amyloid precursor proteins (APP) are metabolized in the brain itself. Secretory forms of APP in a phosphate buffer-soluble fraction were purified from post-mortem human brain by heparin-affinity and ion-exchange chromatography and analyzed by N-terminal amino acid sequencing and SDS polyacrylamide gel electrophoresis/immunoblotting. We found apparently similar multi-isoforms of secretory APP (at 93-97, 105-112 and 123 KDa) to those that we have described recently in cerebrospinal fluid. Antisera to the initial part of the beta/A4 sequence labelled only those bands that were found to react with antiserum to the Kunitz-type inhibitor insert of APP, suggesting that beta/A4 amyloid may be generated specifically from APP-695.
| Item Type: | Article |
|---|---|
| Journal or Publication Title: | Biochemical and Biophysical Research Communications |
| Uncontrolled Keywords: | Adult ; Aged ; Alzheimer Disease ; Amino Acid Sequence ; Amyloid ; Amyloid beta-Protein Precursor ; Brain ; Down Syndrome ; Female ; Humans ; Male ; Middle Aged ; Molecular Sequence Data |
| Subjects: | UNSPECIFIED |
| Departments: | Faculty of Health and Medicine > Biomedical & Life Sciences |
| ID Code: | 50847 |
| Deposited By: | ep_importer_pure |
| Deposited On: | 08 Nov 2011 10:17 |
| Refereed?: | Yes |
| Published?: | Published |
| Last Modified: | 26 Jul 2012 19:44 |
| Identification Number: | |
| URI: | http://eprints.lancs.ac.uk/id/eprint/50847 |
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