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Secretory form of Alzheimer amyloid precursor protein 695 in human brain lacks β/A4 amyloid immunoreactivity

Kametani, F and Tanaka, K and Ishii, T and Ikeda, S and Kennedy, H E and Allsop, D (1993) Secretory form of Alzheimer amyloid precursor protein 695 in human brain lacks β/A4 amyloid immunoreactivity. Biochemical and Biophysical Research Communications, 191 (2). pp. 392-398. ISSN 0006-291X

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Abstract

It is not clear how Alzheimer amyloid precursor proteins (APP) are metabolized in the brain itself. Secretory forms of APP in a phosphate buffer-soluble fraction were purified from post-mortem human brain by heparin-affinity and ion-exchange chromatography and analyzed by N-terminal amino acid sequencing and SDS polyacrylamide gel electrophoresis/immunoblotting. We found apparently similar multi-isoforms of secretory APP (at 93-97, 105-112 and 123 KDa) to those that we have described recently in cerebrospinal fluid. Antisera to the initial part of the beta/A4 sequence labelled only those bands that were found to react with antiserum to the Kunitz-type inhibitor insert of APP, suggesting that beta/A4 amyloid may be generated specifically from APP-695.

Item Type: Article
Journal or Publication Title: Biochemical and Biophysical Research Communications
Uncontrolled Keywords: Adult ; Aged ; Alzheimer Disease ; Amino Acid Sequence ; Amyloid ; Amyloid beta-Protein Precursor ; Brain ; Down Syndrome ; Female ; Humans ; Male ; Middle Aged ; Molecular Sequence Data
Subjects:
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 50847
Deposited By: ep_importer_pure
Deposited On: 08 Nov 2011 10:17
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 19:44
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/50847

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