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Human IMR-32 neuroblastoma cells as a model cell line in Alzheimer's disease research

Neill, D and Hughes, D and Edwardson, J A and Rima, B K and Allsop, D (1994) Human IMR-32 neuroblastoma cells as a model cell line in Alzheimer's disease research. Journal of Neuroscience Research, 39 (4). pp. 482-493. ISSN 0360-4012

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Abstract

The present study investigated expression and processing of amyloid precursor protein by neuronally differentiated IMR-32 neuroblastoma cells. APP mRNA in these cells was found to consist of approximately 58% APP695, 38% APP751, and <4% APP770. APP-immunoreactive bands detected in western blots of cellular protein extracts were only detected by anti-APP antibodies to peptides with strong homology to APLP2, suggesting that these bands represent APP-like proteins and not APP itself. This result suggests that previous studies claiming immunodetection of cellular forms of APP may have to be re-evaluated. Four main species of C-terminal truncated, secreted APP were detected in blots of protein extracts from medium conditioned by these cells. The immunoreactive profile of these bands suggested a cleavage site N-terminal to the Lys16-Leu17 bond of alpha-secretase. This, together with differences in number and molecular mass of APP-immunoreactive bands between secreted APP from IMR-32 cells and that from the commonly used PC-12 cells, suggests differences in APP processing between these two neuronally differentiated cell lines. In theory, IMR-32 cells being of human neuronal origin may be a more appropriate cell line to study APP-processing in relation to Alzheimer's disease than the rat phaeochromocytoma PC-12 cell line. Therefore, these detected differences warrant further investigation. Additionally IMR-32 cells under certain tissue culture conditions can form intracellular fibrillary material that reacts with anti-PHF specific antibodies. Neuronally differentiated IMR-32 cells could therefore be used as a model system to investigate possible interactions between APP-processing and PHF formation.

Item Type: Article
Journal or Publication Title: Journal of Neuroscience Research
Uncontrolled Keywords: Alzheimer Disease ; Amino Acid Sequence ; Amyloid beta-Protein Precursor ; Antibodies ; Antibodies, Monoclonal ; Base Sequence ; Blotting, Northern ; Cell Differentiation ; Cell Line ; Humans ; Immunohistochemistry ; Models, Neurological ; Molecular Sequence Data ; Neuroblastoma ; Neurons ; Oligonucleotide Probes ; Peptide Fragments ; RNA, Messenger ; Sequence Homology, Amino Acid ; Tumor Cells, Cultured
Subjects: UNSPECIFIED
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 50836
Deposited By: ep_importer_pure
Deposited On: 07 Nov 2011 16:31
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 19:43
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/50836

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