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The immunoreactive profile at the N-terminal region of Aβ1-39/40 but not Aβ1-42 changes with transition from monomer/dimer to further peptide aggregates

Kametani, F and Tanaka, K and Tokuda, T and Allsop, D (1995) The immunoreactive profile at the N-terminal region of Aβ1-39/40 but not Aβ1-42 changes with transition from monomer/dimer to further peptide aggregates. Brain Research, 703 (1-2). pp. 237-241. ISSN 0006-8993

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Abstract

Using site-specific antibodies, we assessed the effect of aggregation of various length forms of A beta on the immunoreactive profile of the peptides. All of the antibodies tested reacted with monomeric/dimeric forms of A beta 1-42 and its further aggregates. However, antibodies directed against the 1-24 region of A beta reacted weakly or not at all with A beta 1-39/40 monomers or dimers, but immunoreactivity was enhanced substantially following peptide incubation and aggregation. These results suggest that the conformation of the N-terminal region of monomeric and dimeric A beta 1-39/40 is different from that of aggregated forms, whereas the longer A beta 1-42 does not significantly change its N-terminal conformation during beta-sheet fibril formation. These immunochemical results are consistent with previous structural data, and help to explain the differential effects of A beta 1-39/40 and 1-42 on fibril formation in brain.

Item Type: Article
Journal or Publication Title: Brain Research
Uncontrolled Keywords: Blotting, Western ; Immunoenzyme Techniques ; Peptide Fragments ; Protein Conformation
Subjects: UNSPECIFIED
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 50834
Deposited By: ep_importer_pure
Deposited On: 07 Nov 2011 13:56
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 19:43
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/50834

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