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Effect of aluminium on expression and processing of amyloid precursor protein

Neill, D and Leake, A and Hughes, D and Keith, A B and Taylor, G A and Allsop, D and Rima, B K and Morris, C and Candy, J M and Edwardson, J A (1996) Effect of aluminium on expression and processing of amyloid precursor protein. Journal of Neuroscience Research, 46 (4). pp. 395-403. ISSN 0360-4012

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Abstract

The environmental agent aluminium has been extensively investigated for a potential role in the aetiology of Alzheimer's disease. Despite many investigations there is at present no definite proof for any involvement. If aluminium is involved it is possible that its action is mediated through interaction with the synthesis or processing of amyloid precursor protein (APP). The present study compared aluminium loaded IMR-32 neuroblastoma cells and rat brains with control cells and brains to determine if aluminium affected APP expression and/or processing. In the IMR-32 model system aluminium had no effect on steady-state APP mRNA levels or on the ratio of individual isoforms. It also had no quantitative or qualitative effect on APP-immunoreactive bands detected in protein extracts from conditioned medium of these cells. In total cell extracts, aluminium reduced the intensity of APP-immunoreactive bands between 120-105 kDa but had no effect on a 9 kDa band. In rat brains, aluminium had no effect on APP-immunoreactive bands from soluble or insoluble-membranous extracts. The results, in general, provide no evidence for any effect of aluminium on APP expression or processing.

Item Type: Article
Journal or Publication Title: Journal of Neuroscience Research
Uncontrolled Keywords: Aluminum ; Alzheimer Disease ; Amyloid beta-Protein Precursor ; Animals ; Apoproteins ; Blotting, Northern ; Blotting, Western ; Brain Chemistry ; Gene Expression Regulation ; Humans ; Molecular Weight ; Nerve Tissue Proteins ; Neuroblastoma ; Protein Processing, Post-Translational ; RNA, Messenger ; Rats ; Transferrin ; Tumor Cells, Cultured
Subjects: UNSPECIFIED
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 50832
Deposited By: ep_importer_pure
Deposited On: 07 Nov 2011 13:13
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 19:43
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/50832

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