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Distinct sites of intracellular production for Alzheimer's disease Aβ40/42 amyloid peptides

Hartmann, T and Bieger, S C and Brühl, B and Tienari, P J and Ida, N and Allsop, D and Roberts, G W and Masters, C L and Dotti, C G and Unsicker, K and Beyreuther, K (1997) Distinct sites of intracellular production for Alzheimer's disease Aβ40/42 amyloid peptides. Nature Medicine, 3 (9). pp. 1016-1020. ISSN 1078-8956

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Abstract

The Alzheimer amyloid precursor protein (APP) is cleaved by several proteases, the most studied, but still unidentified ones, are those involved in the release of a fragment of APP, the amyloidogenic beta-protein A beta. Proteolysis by gamma-secretase is the last processing step resulting in release of A beta. Cleavage occurs after residue 40 of A beta [A beta(1-40)], occasionally after residue 42 [A beta(1-42)]. Even slightly increased amounts of this A beta(1-42) might be sufficient to cause Alzheimer's disease (AD) (reviewed in ref. 1, 2). It is thus generally believed that inhibition of this enzyme could aid in prevention of AD. Unexpectedly we have identified in neurons the endoplasmic reticulum (ER) as the site for generation of A beta(1-42) and the trans-Golgi network (TGN) as the site for A beta(1-40) generation. It is interesting that intracellular generation of A beta seemed to be unique to neurons, because we found that nonneuronal cells produced significant amounts of A beta(1-40) and A beta(1-42) only at the cell surface. The specific production of the critical A beta isoform in the ER of neurons links this compartment with the generation of A beta and explains why primarily ER localized (mutant) proteins such as the presenilins could induce AD. We suggest that the earliest event taking place in AD might be the generation of A beta(1-42) in the ER.

Item Type: Article
Journal or Publication Title: Nature Medicine
Uncontrolled Keywords: Alzheimer Disease ; Amyloid Precursor Protein Secretases ; Amyloid beta-Peptides ; Animals ; Aspartic Acid Endopeptidases ; COS Cells ; Cell Compartmentation ; Cell Membrane ; Endopeptidases ; Endoplasmic Reticulum ; Golgi Apparatus ; Hippocampus ; Humans ; Microscopy, Immunoelectron ; Neurons ; Peptide Fragments ; Rats
Subjects: UNSPECIFIED
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 50831
Deposited By: ep_importer_pure
Deposited On: 07 Nov 2011 13:10
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 19:43
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/50831

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