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Phosphorylated α-synuclein can be detected in blood plasma and is potentially a useful biomarker for Parkinson's disease

Foulds, Penny and Mitchell, J Douglas and Parker, Angela and Turner, Roisin and Green, Gerwyn and Diggle, Peter and Hasegawa, Masato and Taylor, Mark and Mann, David and Allsop, David (2011) Phosphorylated α-synuclein can be detected in blood plasma and is potentially a useful biomarker for Parkinson's disease. The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 25 (12). pp. 4127-4137. ISSN 0892-6638

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Abstract

Parkinson's disease (PD) is characterized by the presence of Lewy bodies containing phosphorylated and aggregated α-synuclein (α-syn). α-Syn is present in human body fluids, including blood plasma, and is a potential biomarker for PD. Immunoassays for total and oligomeric forms of both normal and phosphorylated (at Ser-129) α-syn have been used to assay plasma samples from a longitudinal cohort of 32 patients with PD (sampled at mo 0, 1, 2, 3), as well as single plasma samples from a group of 30 healthy control participants. The levels of α-syn in plasma varied greatly between individuals, but were remarkably consistent over time within the same individual with PD. The mean level of phospho-α-syn was found to be higher (P=0.053) in the PD samples than the controls, whereas this was not the case for total α-syn (P=0.244), oligo-α-syn (P=0.221), or oligo-phospho-α-syn (P=0.181). Immunoblots of plasma revealed bands (at 21, 24, and 50-60 kDa) corresponding to phosphorylated α-syn. Thus, phosphorylated α-syn can be detected in blood plasma and shows more promise as a diagnostic marker than the nonphosphorylated protein. Longitudinal studies undertaken over a more extended time period will be required to determine whether α-syn can act as a marker of disease progression.-Foulds, P. G., Mitchell, J. D., Parker, A., Turner, R., Green, G., Diggle, P., Hasegawa, M., Taylor, M., Mann, D., Allsop, D. Phosphorylated α-synuclein can be detected in blood plasma and is potentially a useful biomarker for Parkinson's disease.

Item Type: Article
Journal or Publication Title: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Uncontrolled Keywords: Lewy body ; oligomer ; immunoassay ; immunoblot
Subjects: R Medicine > R Medicine (General)
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
Faculty of Health and Medicine
Faculty of Health and Medicine > Medicine
ID Code: 50827
Deposited By: ep_importer_pure
Deposited On: 07 Nov 2011 10:15
Refereed?: Yes
Published?: Published
Last Modified: 17 Sep 2013 08:27
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/50827

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