Lancaster EPrints

Fucose content of keratan sulphates from bovine articular cartilage

Tai, G H and Brown, G M and Morris, H G and Huckerby, T N and Nieduszynski, I A (1991) Fucose content of keratan sulphates from bovine articular cartilage. Biochemical Journal, 273 (2). pp. 307-310. ISSN 0264-6021

Full text not available from this repository.

Abstract

Alkaline-borohydride-reduced keratan sulphate chains were isolated from bovine articular cartilage (6-8-year-old animals). Nine keratan sulphate fractions of increasing molecular weight were prepared by gel-permeation chromatography on a calibrated column of TSK 30 XL. The samples were analysed for fucose and galactose contents (% by wt. of keratan sulphate) and fucose/galactose ratio. The fucose content increased with molecular size, but the galactose content remained constant. It was concluded that the alpha(1----3)-linked fucose [Thornton, Morris, Cockin, Huckerby, Nieduszynski, Carlstedt, Hardingham & Ratcliffe (1989) Biochem. J. 260, 277-282] was located within the poly-N-acetyl-lactosamine repeat sequence of articular-cartilage keratan sulphate.

Item Type: Article
Journal or Publication Title: Biochemical Journal
Uncontrolled Keywords: Animals ; Borohydrides ; Carbohydrates ; Cartilage, Articular ; Cattle ; Chromatography, Gel ; Fucose ; Galactose ; Keratan Sulfate ; Magnetic Resonance Spectroscopy ; Molecular Weight
Subjects: UNSPECIFIED
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
VC's Office
ID Code: 50616
Deposited By: ep_importer_pure
Deposited On: 01 Nov 2011 13:52
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 19:41
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/50616

Actions (login required)

View Item