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13C-NMR spectroscopy of keratan sulphates--assignments for five sialylated pentasaccharides derived from the non-reducing chain termini of bovine articular cartilage keratan sulphate by keratanase II digestion

Huckerby, T N and Brown, Gavin and Nieduszynski, I A (1998) 13C-NMR spectroscopy of keratan sulphates--assignments for five sialylated pentasaccharides derived from the non-reducing chain termini of bovine articular cartilage keratan sulphate by keratanase II digestion. European Journal of Biochemistry, 251 (3). pp. 991-997. ISSN 0014-2956

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Abstract

Skeletal keratan sulphate has been fragmented using the enzyme keratanase II, and 13C chemical-shift data are reported for five reduced sialylated pentasaccharides that derived from the non-reducing chain terminal region. They have the structures: NeuAc(alpha2-6)Gal(beta1-4)GlcNAc6S(beta1-3)Gal(beta1-4)GlcNAc6 S-ol, NeuAc(alpha2-3)Gal(beta1-4)GlcNAc6S(beta1-3)Gal(beta1-4)GlcNAc6 S-ol, NeuAc(alpha2-6)Gal(beta1-4)GlcNAc6S(beta1-3)Gal6S(beta1-4)++ +GlcNAc6S-ol, NeuAc(alpha2-3)Gal(beta1-4)GlcNAc6S(beta1-3)Gal(6S)(beta1-4)Glc NAc6S-ol, and NeuAc(alpha2-3)Gal(6S)(beta1-4)GlcNAc6S(beta1-3)Gal(6S)(beta1-4)++ +GlcNAc6S-ol, where GlcNAc6S-ol represents N-acetyl-glucosaminitol 6-O-sulphate and NeuAc represents N-acetylneuraminic acid. The use of these 13C-NMR spectroscopy data for the recognition of specific chain-capping structures within native keratan sulphates is discussed. In addition, examination of the data derived from the NeuAc(alpha2-6) capping structures strongly suggests that sulphation three residues away from the neuraminic acid cap has a profound effect upon the conformation of the capping region.

Item Type: Article
Journal or Publication Title: European Journal of Biochemistry
Uncontrolled Keywords: Acetylglucosaminidase ; Animals ; Carbohydrate Conformation ; Carbohydrate Sequence ; Carbon Isotopes ; Cartilage, Articular ; Cattle ; Keratan Sulfate ; Molecular Sequence Data ; Nuclear Magnetic Resonance, Biomolecular ; Oligosaccharides
Subjects:
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
VC's Office
ID Code: 50613
Deposited By: ep_importer_pure
Deposited On: 01 Nov 2011 13:38
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 19:41
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/50613

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