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Human aggrecan keratan sulfate undergoes structural changes during adolescent development

Brown, Gavin and Huckerby, T N and Bayliss, M T and Nieduszynski, I A (1998) Human aggrecan keratan sulfate undergoes structural changes during adolescent development. Journal of Biological Chemistry, 273 (41). pp. 26408-26414. ISSN 0021-9258

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Abstract

Alkaline borohydride-reduced keratan sulfate chains were isolated from human articular cartilage aggrecan from individuals of various ages (0-85 years old). The chains were structurally characterized using 1H NMR spectroscopy, gel permeation chromatography, and oligosaccharide profiling (after digestion with the enzymes keratanase and keratanase II). The results show that from birth to early adolescence (0-9 years) the levels of alpha(1-3)-fucosylation, alpha(2-3)-sialylation, and galactose sulfation increase. Also, the weight-average molecular weight of the chains increases. During maturation (9-18 years) the levels of fucosylation and galactose sulfation continue to increase and alpha(2-6)-sialylation of the chains occurs. In adult life (18-85 years) there is little change in the weight-average molecular weight of the chains, and the levels of fucosylation, sialylation, and sulfation remain fairly constant.

Item Type: Article
Journal or Publication Title: Journal of Biological Chemistry
Uncontrolled Keywords: Acetylglucosaminidase ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Aggrecans ; Carbohydrate Conformation ; Carbohydrate Sequence ; Extracellular Matrix Proteins ; Fucose ; Humans ; Hydrolysis ; Keratan Sulfate ; Lectins, C-Type ; Magnetic Resonance Spectroscopy ; Middle Aged ; Molecular Sequence Data ; N-Acetylneuraminic Acid ; Proteoglycans
Subjects: UNSPECIFIED
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
VC's Office
ID Code: 50612
Deposited By: ep_importer_pure
Deposited On: 01 Nov 2011 13:34
Refereed?: Yes
Published?: Published
Last Modified: 01 Oct 2013 11:04
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/50612

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