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Analysis of ESTs from Lutzomyia longipalpis sand flies and their contribution toward understanding the insect-parasite relationship

Dillon, Rod J. and Ivens, Al C. and Churcher, Carol and Holroyd, Nancy and Quail, Michael A. and Rogers, Matthew E. and Soares, M. Bento and Bonaldo, Maria F. and Casavant, Thomas L. and Lehane, Mike J. and Bates, Paul A. (2006) Analysis of ESTs from Lutzomyia longipalpis sand flies and their contribution toward understanding the insect-parasite relationship. Genomics, 88 (6). pp. 831-840. ISSN 0888-7543

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Abstract

An expressed sequence tag library has been generated from a sand fly vector of visceral leishmaniasis, Lutzomyia longipalpis. A normalized cDNA library was constructed from whole adults and 16,608 clones were sequenced from both ends and assembled into 10,203 contigs and singlets. Of these 58% showed significant similarity to known genes from other organisms, < 4% were identical to described sand fly genes, and 42% had no match to any database sequence. Our analyses revealed putative proteins involved in the barrier function of the gut (pelitrophins, microvillar proteins, glutamine synthase), digestive physiology (secreted and membrane-anchored hydrolytic enzymes), and the immune response (gram-negative binding proteins, thioester proteins, scavenger receptors, galectins, signating pathway factors, caspases, serpins, and peroxidases). Sequence analysis of this transcriptome dataset has provided new insights into genes that might be associated with the response of the vector to the development of Leishmania.

Item Type: Article
Journal or Publication Title: Genomics
Uncontrolled Keywords: Lutzomyia ; expressed sequence tag ; Leishmania ; genomics ; immunity ; parasite ; midgut ; PHLEBOTOMUS-PAPATASI DIPTERA ; GLOSSINA-MORSITANS-MORSITANS ; MOSQUITO ANOPHELES-GAMBIAE ; EXPRESSED SEQUENCE TAGS ; AEDES-AEGYPTI ; IMMUNE-RESPONSE ; MOLECULAR CHARACTERIZATION ; PLASMODIUM-BERGHEI ; MIDGUT CELLS ; LEISHMANIA-MEXICANA
Subjects: Q Science > QR Microbiology > QR355 Virology
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 50568
Deposited By: ep_importer_pure
Deposited On: 29 Oct 2011 00:21
Refereed?: Yes
Published?: Published
Last Modified: 06 Aug 2012 09:10
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/50568

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