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cGMP-dependent ABA-induced stomatal closure in the ABA-insensitive Arabidopsis mutant abi1-1

Dubovskaya, Lyudmila V. and Bakakina, Yulia S. and Kolesneva, Ekaterina V. and Sodel, Dmitry L. and McAinsh, Martin R. and Hetherington, Alistair M. and Volotovski, Igor D. (2011) cGMP-dependent ABA-induced stomatal closure in the ABA-insensitive Arabidopsis mutant abi1-1. New Phytologist, 191 (1). pp. 57-69. ISSN 0028-646X

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Abstract

The drought hormone abscisic acid (ABA) is widely known to produce reductions in stomatal aperture in guard cells. The second messenger cyclic guanosine 3', 5'-monophosphate (cGMP) is thought to form part of the signalling pathway by which ABA induces stomatal closure. We have examined the signalling events during cGMP-dependent ABA-induced stomatal closure in wild-type Arabidopsis plants and plants of the ABA-insensitive Arabidopsis mutant abi1-1. We show that cGMP acts downstream of hydrogen peroxide (H2O2) and nitric oxide (NO) in the signalling pathway by which ABA induces stomatal closure. H2O2- and NO-induced increases in the cytosolic free calcium concentration ([Ca2+](cyt)) were cGMP-dependent, positioning cGMP upstream of [Ca2+](cyt), and involved the action of the type 2C protein phosphatase ABI1. Increases in cGMP were mediated through the stimulation of guanylyl cyclase by H2O2 and NO. We identify nucleoside diphosphate kinase as a new cGMP target protein in Arabidopsis. This study positions cGMP downstream of ABA-induced changes in H2O2 and NO, and upstream of increases in [Ca2+](cyt) in the signalling pathway leading to stomatal closure.

Item Type: Article
Journal or Publication Title: New Phytologist
Uncontrolled Keywords: abi1-1 ; abscisic acid (ABA) ; Arabidopsis ; cyclic guanosine 3 ',5 '-monophosphate (cGMP) ; guard cells ; second messengers ; stomatal closure ; NUCLEOSIDE DIPHOSPHATE KINASE ; PROTEIN PHOSPHATASE 2C ; OXYGEN SPECIES PRODUCTION ; ABSCISIC-ACID ; GUARD-CELLS ; HYDROGEN-PEROXIDE ; NITRIC-OXIDE ; OXIDATIVE STRESS ; CYCLIC-GMP ; SIGNALING PATHWAYS
Subjects: UNSPECIFIED
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 50325
Deposited By: ep_importer_pure
Deposited On: 12 Oct 2011 11:23
Refereed?: Yes
Published?: Published
Last Modified: 06 Sep 2013 22:45
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/50325

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