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Metabolic rate is not reduced by dietary-restriction or by lowered insulin/IGF-1 signalling and is not correlated with individual lifespan in Drosophila melanogaster

Hulbert, A. J. and Clancy, D. J. and Mair, W. and Braeckman, B. P. and Gems, D. and Partridge, L. (2004) Metabolic rate is not reduced by dietary-restriction or by lowered insulin/IGF-1 signalling and is not correlated with individual lifespan in Drosophila melanogaster. Experimental Gerontology, 39 (8). pp. 1137-1143.

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Abstract

The link between resting metabolic rate and aging, measured as adult lifespan, was investigated in Drosophila melanogaster by (i) comparing lifespan and metabolic rate of individual flies, (ii) examining the effect of dietary-restriction on the metabolic rate of adult flies, and (iii) comparing the metabolic rate of wild-type and insulin/IGF-1 signalling mutant chico(1) flies. The resting oxygen consumption of 65 individually housed and fully fed Drosophila was measured weekly throughout their lifetime. There was no significant difference in the mass-specific rate of oxygen consumption between cohorts that differed in lifespan. Nor was there any statistical correlation between mass-specific oxygen consumption and lifespan of individual Drosophila. The average mass-specific rate of oxygen consmuption at 25degreesC was 3.52 +/- 0.07 mul O-2 mg(-1) h(-1). Variation in mass-specific metabolic rate explained only 4% of variation in individual life span in these flies. Contrary to predictions from the 'rate of living' theory of aging lifetime oxygen consumption was not constant and the lifespan of individual flies accounted for 91% of their lifetime oxygen consumption. An average Drosophila consumes about 3 ml O-2 during its adult life. Dietary-restriction had no effect on mass-specific resting metabolic rate both when measured as oxygen consumption by respirometry and when measured as heat production by microcalorimetry. The mass-specific resting heat production of fully fed adult flies at 25degreesC averaged 17.3 +/- 0.3 muW mg(-1). Similarly there was no difference in mass-specific metabolic rate of wild-type flies and longliving chico(1) insulin/IGF-1 signalling mutant flies, either when measured as oxygen consumption or heat production. Thus, individual variation in lifespan in wild-type flies, and life extension by dietary-restriction and reduced insulin/IGF-1 signalling is not attributable to differences in metabolic rate. (C) 2004 Elsevier Inc. All rights reserved.

Item Type: Article
Journal or Publication Title: Experimental Gerontology
Additional Information: Aug Metabolic rate is not reduced by dietary-restriction or by lowered insulin/IGF-1 signalling and is not correlated with individual lifespan in Drosophila melanogaster
Uncontrolled Keywords: Drosophila melanogaster ; Metabolic rate ; Dietary-restriction
Subjects: UNSPECIFIED
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 50155
Deposited By: ep_importer_pure
Deposited On: 30 Sep 2011 16:28
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 19:36
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/50155

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